Health Nutrition, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Nutrients. 2022 Apr 18;14(8):1676. doi: 10.3390/nu14081676.
Epidemiological studies have suggested that coffee consumption is associated with a decrease in the risk of developing obesity and diabetes; however, the detailed mechanisms underlying these effects of coffee consumption remain poorly understood. In this study, we examined the effects of chlorogenic acid on energy metabolism in vitro. Hepatocellular carcinoma G2 (HepG2) cells were cultured in a medium containing chlorogenic acid. Chlorogenic acid increased the activity of mitochondrial enzymes, including citrate synthase, isocitrate dehydrogenase, and malate dehydrogenase (MDH), which are involved in the tricarboxylic acid (TCA) cycle. Proteome analysis using the isobaric tags for the relative and absolute quantitation (iTRAQ) method revealed the upregulation of proteins involved in the glycolytic system, electron transport system, and ATP synthesis in mitochondria. Therefore, we propose a notable mechanism whereby chlorogenic acid enhances energy metabolism, including the TCA cycle, glycolytic system, electron transport, and ATP synthesis. This mechanism provides important insights into understanding the beneficial effects of coffee consumption.
流行病学研究表明，咖啡的摄入与肥胖和糖尿病风险的降低有关；然而，咖啡摄入的这些作用的详细机制仍知之甚少。在这项研究中，我们研究了绿原酸对体外能量代谢的影响。肝癌细胞 G2（HepG2）在含有绿原酸的培养基中培养。绿原酸增加了柠檬酸合酶、异柠檬酸脱氢酶和苹果酸脱氢酶（MDH）等参与三羧酸（TCA）循环的线粒体酶的活性。采用相对和绝对定量同位素标记（iTRAQ）方法的蛋白质组分析显示，参与糖酵解系统、电子传递系统和线粒体 ATP 合成的蛋白质上调。因此，我们提出了一个显著的机制，即绿原酸增强了能量代谢，包括 TCA 循环、糖酵解系统、电子传递和 ATP 合成。该机制为理解咖啡摄入的有益作用提供了重要的见解。
