Querido Diana, Vieira Tânia, Ferreira José Luís, Henriques Célia, Borges João Paulo, Silva Jorge Carvalho
Cenimat/I3N, Physics Department, School of Science and Technology, Universidade Nova de Lisboa, Caparica, 2819-516 Almada, Portugal.
Cenimat/I3N, Materials Science Department, School of Science and Technology, Universidade Nova de Lisboa, Caparica, 2819-516 Almada, Portugal.
Polymers (Basel). 2022 Apr 7;14(8):1496. doi: 10.3390/polym14081496.
Hybrid scaffolds obtained by combining two or more biopolymers are studied in the context of tissue regeneration due to the possibility of achieving new functional properties or structural features. The aim of this work was to produce a new type of hybrid polycaprolactone (PCL)/chitosan (CS) electrospun mat through the controlled deposition of CS flakes interspaced between the PCL fibers. A poly(ethylene oxide) (PEO) solution was used to transport CS flakes with controlled size. This, and the PCL solution, were simultaneously electrospun onto a rotatory mandrel in a perpendicular setup. Different PCL/CS mass ratios were also studied. The morphology of the resulting fibers, evaluated by SEM, confirmed the presence of the CS flakes between the PCL fibers. The addition of PEO/CS fibers resulted in hydrophilic mats with lower Young's modulus relatively to PCL mats. In vitro cell culture results indicated that the addition of CS lowers both the adhesion and the proliferation of human dermal fibroblasts. The present work demonstrates the feasibility of achieving a controlled deposition of a polymeric component in granular form onto a collector where electrospun nanofibers are being deposited, thereby producing a hybrid scaffold.
由于有可能获得新的功能特性或结构特征，因此在组织再生的背景下研究了通过组合两种或更多种生物聚合物获得的混合支架。这项工作的目的是通过在聚己内酯（PCL）纤维之间间隔控制沉积壳聚糖（CS）薄片来制备一种新型的混合PCL/CS电纺垫。使用聚环氧乙烷（PEO）溶液来输送尺寸可控的CS薄片。将其与PCL溶液在垂直设置下同时电纺到旋转心轴上。还研究了不同的PCL/CS质量比。通过扫描电子显微镜（SEM）评估所得纤维的形态，证实了PCL纤维之间存在CS薄片。添加PEO/CS纤维导致亲水性垫，其杨氏模量相对于PCL垫较低。体外细胞培养结果表明，添加CS会降低人皮肤成纤维细胞的粘附和增殖。本工作证明了将颗粒形式的聚合物组分可控沉积到正在沉积电纺纳米纤维的收集器上的可行性，从而制备出混合支架。
