Chiu Chun-Hui, Chiu Kai-Chu, Yang Li-Chan
Graduate Institute of Health Industry and Technology, Research Center for Chinese Herbal Medicine and Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan City 333, Taiwan.
Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung 204, Taiwan.
Polymers (Basel). 2022 Apr 8;14(8):1514. doi: 10.3390/polym14081514.
Obesity is rapidly becoming an emerging disease in developing countries due to the Westernization of societies and lifestyle changes. This study evaluated the ameliorative effect of acidic heteropolysaccharides derived from (TFPS) on high-fat diet (HFD; 34.9% fat)-induced obesity in mice. The TFPS exhibited high uronic acid content and high viscosity in water. The structural characteristics of TFPS showed that average molecular weight was 679 kDa, and the monosaccharide composition was galactose, glucose, fructose, xylose, fucose, and mannose at a ratio of 1.0:6.5:10.0:18.5:30.5:67.5. In an in vivo study, HFD-induced obese C57BL/6 mice were orally given a TFPS treatment at 1 and 2 g/kg of body weight for 8 weeks. The TFPS treatment significantly reduced features of obesity in the mice, namely weight gain, feed efficiency, body fat percentage, and serum cholesterol level and increased fecal lipid content, compared with mice fed an HFD with water. In addition, TFPS exhibited the inhibition of cholesterol micelles in vitro in a concentration-dependent manner. In conclusion, the TFPS treatment ameliorated the diet-induced obesity in the mice, presumably reducing fat absorption in the intestine by interfering with viscous TFPS.
由于社会的西方化和生活方式的改变，肥胖在发展中国家正迅速成为一种新兴疾病。本研究评估了从[具体来源未给出]提取的酸性杂多糖（TFPS）对高脂饮食（HFD；脂肪含量34.9%）诱导的小鼠肥胖的改善作用。TFPS在水中表现出高糖醛酸含量和高粘度。TFPS的结构特征表明其平均分子量为679 kDa，单糖组成为半乳糖、葡萄糖、果糖、木糖、岩藻糖和甘露糖，比例为1.0:6.5:10.0:18.5:30.5:67.5。在一项体内研究中，给HFD诱导的肥胖C57BL/6小鼠口服1和2 g/kg体重的TFPS，持续8周。与喂水的HFD小鼠相比，TFPS处理显著降低了小鼠的肥胖特征，即体重增加、饲料效率、体脂百分比和血清胆固醇水平，并增加了粪便脂质含量。此外，TFPS在体外以浓度依赖的方式抑制胆固醇微团。总之，TFPS处理改善了饮食诱导的小鼠肥胖，可能是通过干扰粘性的TFPS减少了肠道脂肪吸收。
