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氘标记维生素 D 代谢物的合成作为 LC-MS 分析的内标。

Synthesis of Deuterium-Labeled Vitamin D Metabolites as Internal Standards for LC-MS Analysis.

机构信息

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.

Medical Equipment Business Operations, Management Strategy Planning Division, JEOL Ltd., Akishima, Tokyo 196-8558, Japan.

出版信息

Molecules. 2022 Apr 9;27(8):2427. doi: 10.3390/molecules27082427.

DOI:10.3390/molecules27082427
PMID:35458625
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9025501/
Abstract

Blood levels of the vitamin D (D) metabolites 25-hydroxyvitamin D (25(OH)D), 24,25-dihydroxyvitamin D, and 1α,25-dihydroxyvitamin D (1,25(OH)D) are recognized indicators for the diagnosis of bone metabolism-related diseases, D deficiency-related diseases, and hypercalcemia, and are generally measured by liquid-chromatography tandem mass spectrometry (LC-MS/MS) using an isotope dilution method. However, other D metabolites, such as 20-hydroxyvitamin D and lactone D, also show interesting biological activities and stable isotope-labeled derivatives are required for LC-MS/MS analysis of their concentrations in serum. Here, we describe a versatile synthesis of deuterium-labeled D metabolites using A-ring synthons containing three deuterium atoms. Deuterium-labeled 25(OH)D (), 25(OH)D-23,26-lactone (), and 1,25(OH)D-23,26-lactone () were synthesized, and successfully applied as internal standards for the measurement of these compounds in pooled human serum. This is the first quantification of 1,25(OH)D-23,26-lactone () in human serum.

摘要

血液中维生素 D（D）代谢物 25-羟维生素 D（25(OH)D）、24,25-二羟维生素 D 和 1α,25-二羟维生素 D（1,25(OH)D）的水平被认为是诊断与骨代谢相关的疾病、D 缺乏相关疾病和高钙血症的指标，通常采用液相色谱-串联质谱法（LC-MS/MS）并用同位素稀释法进行测量。然而，其他 D 代谢物，如 20-羟维生素 D 和内酯 D，也表现出有趣的生物活性，需要对其在血清中的浓度进行 LC-MS/MS 分析时使用稳定同位素标记的衍生物。在这里，我们描述了一种使用含有三个氘原子的 A 环合成子合成氘标记 D 代谢物的通用方法。合成了氘标记的 25(OH)D（）、25(OH)D-23,26-内酯（）和 1,25(OH)D-23,26-内酯（），并成功地将其用作这些化合物在混合人血清中测量的内标。这是首次对人血清中 1,25(OH)D-23,26-内酯（）进行定量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/3f6efb0f006a/molecules-27-02427-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/ca92ff8ddccd/molecules-27-02427-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/eaeacf7f0127/molecules-27-02427-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/96445efc2d89/molecules-27-02427-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/b56a4ee87403/molecules-27-02427-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/e6d1f09334e4/molecules-27-02427-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/c85c735ca69e/molecules-27-02427-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/c1dba0423650/molecules-27-02427-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/3f6efb0f006a/molecules-27-02427-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/ca92ff8ddccd/molecules-27-02427-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/eaeacf7f0127/molecules-27-02427-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/96445efc2d89/molecules-27-02427-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/b56a4ee87403/molecules-27-02427-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/e6d1f09334e4/molecules-27-02427-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/c85c735ca69e/molecules-27-02427-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/c1dba0423650/molecules-27-02427-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ede/9025501/3f6efb0f006a/molecules-27-02427-g005.jpg

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