Dipartimento di Scienze e Tecnologie Ambientali, Biologiche, Farmaceutiche, Università della Campania "Luigi Vanvitelli", Via Vivaldi 43, 81100 Caserta, Italy.
CEINGE, Biotecnologie Avanzate Scarl, Via Gaetano Salvatore 486, 80145 Naples, Italy.
Molecules. 2022 Apr 15;27(8):2566. doi: 10.3390/molecules27082566.
Cannabidiolic acid (CBDA) is the main precannabinoid in industrial hemp. It represents a common constituent of hemp seed oil, but mainly abundant in the aerial parts of the plant (including their processing waste). Thus, the optimization of fast and low-cost purification strategies is mandatory, as well as a deep investigation on its nutraceutical and cosmeceutical properties. To this purpose, CBDA content in hemp seed oil is evaluated, and its recovery from wasted leaves is favorably achieved. The cytotoxicity screening towards HaCaT cells, by means of MTT, SRB and LDH release assays, suggested it was not able to decrease cell viability or perturb cell integrity up to 10 μM concentration. Thus, the ability of CBDA to differentially modulate the release of proinflammatory cytokines and chemokines mediators has been evaluated, finding that CBDA decreased IFN-γ, CXCL8, CXCL10, CCL2, CCL4 and CCL5, mostly in a dose-dependent manner, with 10 μM tested concentration exerting the highest activity. These data, together with those from assessing antimicrobial activity against Gram(+) and Gram(-) bacteria and the antibiofilm formation, suggest that CBDA is able to counteract the inflammatory response, also preventing bacteria colonization.
大麻二酚酸（CBDA）是工业大麻中的主要前体大麻素。它是大麻籽油的常见成分，但主要存在于植物的地上部分（包括其加工废物）。因此，优化快速且低成本的纯化策略是强制性的，同时还需要深入研究其营养保健品和化妆品特性。为此，评估了大麻籽油中的 CBDA 含量，并从废弃的叶子中有利地回收了 CBDA。通过 MTT、SRB 和 LDH 释放测定法对 HaCaT 细胞进行的细胞毒性筛选表明，它在 10 μM 浓度下不会降低细胞活力或破坏细胞完整性。因此，评估了 CBDA 调节促炎细胞因子和趋化因子介质释放的能力，发现 CBDA 可降低 IFN-γ、CXCL8、CXCL10、CCL2、CCL4 和 CCL5 的释放，主要呈剂量依赖性，在 10 μM 测试浓度下具有最高的活性。这些数据，连同评估对革兰氏阳性菌和革兰氏阴性菌的抗菌活性和抗生物膜形成的数据一起，表明 CBDA 能够抵抗炎症反应，还能防止细菌定植。
