Laboratory of Drug Sciences, Higher Institute of Health Sciences (INSSA), Nazi BONI University, Bobo-Dioulasso 01 P.O. Box 1091, Burkina Faso.
RD3-Pharmacognosy, Bioanalysis and Drug Discovery Unit and Analytical Platform, Faculty of Pharmacy, Université Libre de Bruxelles (ULB), 1050 Brussels, Belgium.
Molecules. 2022 Apr 16;27(8):2583. doi: 10.3390/molecules27082583.
Although is a plant widely used in traditional medicine in sub-Saharan Africa, its biological properties have been poorly investigated to date. We first characterized by conventional reactions the composition of several stem bark extracts and evaluated in acellular and cellular assays their pro- or antioxidant properties supported by their high phenolic and flavonoid content, particularly with the methanolic extract. The pro- or antioxidant effects observed did not correlate with their IC concentrations against five cancer cell lines determined by MTT assay. Indeed, the CHCl extract and its ethyl acetate (EtOAc) subfraction appeared more potent although they harbored lower pro- or antioxidant effects. Nevertheless, at equipotent concentration, both extracts induced ER- and mitochondria-derived vacuoles observed by fluorescent microscopy that further led to non-apoptotic cell death. LC coupled to high resolution MS investigations have been performed to identify chemical compounds of the extracts. These investigations highlighted the presence of compounds formerly isolated from including senegalensein that could be retrieved only in the EtOAc subfraction but also thirteen other compounds, such as 16:3-Glc-stigmasterol and hexadecanoic acid, whose anticancer properties have been previously reported. Nineteen other compounds remain to be identified. In conclusion, appeared rich in compounds with antioxidant and anticancer properties, supporting its use in traditional practice and its status as a species of interest for further investigations in anticancer drug research.
尽管 是一种在撒哈拉以南非洲传统医学中广泛使用的植物，但迄今为止，其生物学特性仍未得到充分研究。我们首先通过常规反应对几种茎皮提取物的组成进行了表征，并通过其高酚类和类黄酮含量（尤其是用甲醇提取物）在非细胞和细胞测定中评估了其促氧化剂或抗氧化剂特性。观察到的促氧化剂或抗氧化剂作用与其对五种癌细胞系的 MTT 测定确定的 IC 浓度无关。实际上，CHCl 提取物及其乙酸乙酯（EtOAc）亚组分尽管表现出较低的促氧化剂或抗氧化剂作用，但似乎更有效。然而，在等效浓度下，两种提取物均诱导荧光显微镜下观察到的 ER 和线粒体衍生的空泡，进一步导致非凋亡性细胞死亡。进行了 LC 与高分辨率 MS 联合调查，以鉴定提取物中的化合物。这些研究强调了提取物中存在以前从 中分离出的化合物，包括仅在 EtOAc 亚组分中可回收的 senegalensein 以及其他 13 种化合物，如 16:3-Glc-豆甾醇和十六烷酸，其抗癌特性以前已有报道。还有 19 种化合物有待鉴定。总之， 富含具有抗氧化和抗癌特性的化合物，支持其在传统实践中的使用及其作为进一步研究抗癌药物研究的有价值物种的地位。
