Zhou Hui, Yao Binghua, Qian Lulu, Hu Haozhong, Duan Qingning
Department of Pediatrics, Taizhou People's Hospital, Taizhou, China.
Department of Pediatrics, Taizhou People's Hospital, Taizhou, China;, Email:
Pharmazie. 2022 Apr 10;77(3):121-124. doi: 10.1691/ph.2022.1872.
Radiotherapy is a common treatment for lung cancer. However, radiation pneumonitis caused by radiotherapy can affect the quality of life and prognosis of lung cancer patients. miR-513a-3p has been found to sensitize human lung adenocarcinoma cells to chemotherapy by targeting glutathione S-transferase P1 (GSTP1). Here, we found that x-ray induced the apoptosis of BEAS-2B and miR-513a-3p expression in a dose- and time-dependent manner, and miR-513a-3p-mimic significantly increased x-ray induced apoptosis, while miR-513a-3p-inhibitor significantly decreased x-ray induced apoptosis. Dual luciferase gene reporter system showed that miR-513a-3p targeted to inhibit the expression of GSTP1 in BEAS-2B cells. Moreover, knockdown of GSTP1 significantly increased, while overexpression of GSTP1 decreased the apoptosis of BEAS-2B induced by x-ray. Importantly, overexpression of GSTP1 significantly reduced miR-513a-3p-mimic elevated x-ray -induced apoptosis in BEAS-2B cells. In conclusion, x-ray caused increased expression of miR-513a-3p, and miR-513a-3p promoted x-ray-induced apoptosis of human lung cells by inhibiting GSTP1.
放射治疗是肺癌的常见治疗方法。然而，放射治疗引起的放射性肺炎会影响肺癌患者的生活质量和预后。已发现miR-513a-3p通过靶向谷胱甘肽S-转移酶P1（GSTP1）使人类肺腺癌细胞对化疗敏感。在此，我们发现X射线以剂量和时间依赖性方式诱导BEAS-2B细胞凋亡和miR-513a-3p表达，并且miR-513a-3p模拟物显著增加X射线诱导的凋亡，而miR-513a-3p抑制剂显著降低X射线诱导的凋亡。双荧光素酶基因报告系统显示miR-513a-3p靶向抑制BEAS-2B细胞中GSTP1的表达。此外，敲低GSTP1显著增加，而GSTP1的过表达降低了X射线诱导的BEAS-2B细胞凋亡。重要的是，GSTP1的过表达显著降低了miR-513a-3p模拟物在BEAS-2B细胞中升高的X射线诱导的凋亡。总之，X射线导致miR-513a-3p表达增加，并且miR-513a-3p通过抑制GSTP1促进X射线诱导的人肺细胞凋亡。
