Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai 600113, India.
Centre for Oncology, Royapettah Government Hospital and Kilpauk Medical College, Chennai 600014, India.
Arch Oral Biol. 2022 Jul;139:105428. doi: 10.1016/j.archoralbio.2022.105428. Epub 2022 Apr 6.
The objective of this study is to identify the association between linc-ROR genetic variants and oral squamous cell carcinoma tumorigenesis.
Four genetic variants of linc-ROR (rs6420545, rs4801078, rs1942348, and rs9636089) were analyzed in 178 OSCCs and 191 controls of the South Indian population by PCR amplification followed by restriction digestion. In addition, we examined whether these variants alter linc-ROR expression levels and the progression of OSCC.
The frequency of linc-ROR rs6420545 and rs4801078 genotypes were significantly associated with advanced tumor grade (>2) (p = 0.002 and p = 0.048), and nodal metastasis (p = 0.001 and p = 0.019), respectively. We observed a significant association of rs6420545 specifically in the over-dominant model [OR 1.77 (95%CI; 1.17-2.68); p = 0.006] and rs9636089 in dominant model [OR 2.17 (95%CI; 1.06 - 4.46); p = 0.03], and allelic model [OR 2.26 (95%CI; 1.13 - 4.53) p = 0.02], respectively. Further, significant upregulation of linc-ROR (p = 0.005) was observed in our cohort, consistent with the HNSCC TCGA dataset (p < 0.0001).
Our findings suggest that the linc-ROR genetic variants could contribute to the metastasis and progression mainly in the late event of tumorigenesis of OSCCs and these variants could be useful in the precision therapeutic management of this cancer particularly in prognosis.
本研究旨在确定 linc-ROR 遗传变异与口腔鳞状细胞癌肿瘤发生之间的关联。
通过聚合酶链反应扩增和限制性消化，分析了印度南部人群中的 178 例口腔鳞状细胞癌和 191 例对照中 linc-ROR 的 4 种遗传变异(rs6420545、rs4801078、rs1942348 和 rs9636089)。此外，我们还研究了这些变体是否改变了 linc-ROR 的表达水平和口腔鳞状细胞癌的进展。
linc-ROR rs6420545 和 rs4801078 基因型的频率与晚期肿瘤分级（>2）（p=0.002 和 p=0.048）和淋巴结转移（p=0.001 和 p=0.019）显著相关。我们观察到 rs6420545 与超显性模型之间存在显著相关性[OR 1.77（95%CI；1.17-2.68）；p=0.006]，rs9636089 与显性模型[OR 2.17（95%CI；1.06-4.46）；p=0.03]和等位基因模型[OR 2.26（95%CI；1.13-4.53）p=0.02]显著相关。此外，在我们的队列中观察到 linc-ROR 的显著上调（p=0.005），这与 HNSCC TCGA 数据集一致（p<0.0001）。
我们的研究结果表明，linc-ROR 遗传变异可能主要导致口腔鳞状细胞癌肿瘤发生的转移和进展，这些变异可能对这种癌症的精准治疗管理，特别是预后具有重要作用。
