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Linc-ROR及其剪接变体2和4在食管鳞状细胞癌中显著上调。

Linc-ROR and its spliced variants 2 and 4 are significantly up-regulated in esophageal squamous cell carcinoma.

作者信息

Sahebi Reza, Malakootian Mahshid, Balalaee Baharak, Shahryari Alireza, Khoshnia Masoud, Abbaszadegan Mohammad Reza, Moradi Abdolvahab, Javad Mowla Seyed

机构信息

Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Basic Med Sci. 2016 Oct;19(10):1131-1135.

Abstract

OBJECTIVES

Similar characteristics of molecular pathways between cellular reprogramming events and tumorigenesis have been accentuated in recent years. Reprogramming-related transcription factors, also known as Yamanaka factors (OCT4, SOX2, KLF4, and c-MYC), are also well-known oncogenes promoting cancer initiation, progression, and cellular transformation into cancer stem cells. Long non-coding RNAs (lncRNAs) are a major class of RNA molecules with emerging roles in stem cell pluripotency, cellular reprogramming, cellular transformation, and tumorigenesis. The long intergenic non-coding RNA ROR (lincRNA-ROR, linc-ROR) acts as a regulator of cellular reprograming through sponging miR-145 that normally negatively regulates the expression of the stemness factors NANOG, OCT4, and SOX2.

MATERIALS AND METHODS

Here, we employed a real-time PCR approach to determine the expression patterns of and its two novel spliced variants (variants 2 and 4) in esophageal squamous cell carcinoma (ESCC).

RESULTS

The quantitative real-time RT-PCR results revealed a significant up-regulation of (=0.0098) and its variants 2 (=0.0250) and 4 (=0.0002) in tumor samples of ESCC, compared to their matched non-tumor tissues obtained from the margin of same tumors. Our data also demonstrated a significant up-regulation of variant 4 in high-grade tumor samples, in comparison to the low-grade ones (=0.04). Moreover, the ROC curve analysis demonstrated that the variant 4 of ROR has a potential to discriminate between tumor and non-tumor samples (=0.66, <0.05).

CONCLUSION

Our data suggest a significant up-regulation of linc-ROR and its variants 2 and 4 in ESCC tissue samples.

摘要

目的

近年来,细胞重编程事件与肿瘤发生之间分子途径的相似特征已得到强化。重编程相关转录因子,也被称为山中因子(OCT4、SOX2、KLF4和c-MYC),也是促进癌症起始、进展以及细胞转化为癌症干细胞的著名癌基因。长链非编码RNA(lncRNA)是一类主要的RNA分子,在干细胞多能性、细胞重编程、细胞转化和肿瘤发生中发挥着新出现的作用。长链基因间非编码RNA ROR(lincRNA-ROR,linc-ROR)通过结合通常对干性因子NANOG、OCT4和SOX2表达起负调控作用的miR-145,充当细胞重编程的调节因子。

材料与方法

在此,我们采用实时PCR方法来确定linc-ROR及其两个新的剪接变体(变体2和4)在食管鳞状细胞癌(ESCC)中的表达模式。

结果

定量实时RT-PCR结果显示,与从同一肿瘤边缘获取的匹配非肿瘤组织相比,ESCC肿瘤样本中linc-ROR(P=0.0098)及其变体变体2(P=0.0250)和4(P=0.0002)显著上调。我们的数据还表明,与低级别肿瘤样本相比,高级别肿瘤样本中变体4显著上调(P=0.04)。此外,ROC曲线分析表明,ROR的变体4有区分肿瘤和非肿瘤样本的潜力(AUC=0.66,P<0.05)。

结论

我们的数据表明ESCC组织样本中linc-ROR及其变体2和4显著上调。

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