Joint Laboratory of Advanced Biomedical Materials (NFU-UGent), Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University (NFU), Nanjing 210037, China.
Joint Laboratory of Advanced Biomedical Materials (NFU-UGent), Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University (NFU), Nanjing 210037, China.
Bioorg Chem. 2022 Jul;124:105792. doi: 10.1016/j.bioorg.2022.105792. Epub 2022 Apr 5.
Herein, a high-performance fluorescence probe, namely H4, based on intramolecular charge transfer (ICT) mechanism was developed. H4 could serve as fluorescent probe for detection acidic pH change in environment with outstanding sensitivity, short response time (<10 s), low hemolysis effect (<0.3%) and excellent photostability (>120 min). H4 exhibits a good linear relationship (R = 0.9901, Y = 22.0409X-58.3397) characteristics in range of pH 2.2-7.4 with pK 4.3. In addition, the probe can be also made as fluorescent test sheets and further used as a portable sensor to enhance the screening speed to achieve detection of H in real-time. Further, H4 determined H in real food samples (milk, shrimps, and scallops) has excellent recoveries (98-105%), which making it of great potential use in food freshness evaluation. Initially, its favorable behavior for detecting H change is successfully illustrated in living onion tissues and zebrafish, which allows qualitative visualization of acid pH changes in in biological systems.
本文开发了一种基于分子内电荷转移（ICT）机制的高性能荧光探针 H4。H4 可作为荧光探针，用于检测环境中酸性 pH 值的变化，具有出色的灵敏度、短的响应时间（<10 s）、低的溶血效应（<0.3%）和优异的光稳定性（>120 min）。H4 在 pH 2.2-7.4 范围内表现出良好的线性关系（R = 0.9901，Y = 22.0409X-58.3397），pK 4.3。此外，该探针还可制成荧光测试片，并进一步用作便携式传感器，以提高筛选速度，实现实时检测 H。此外，H4 对实际食品样品（牛奶、虾和扇贝）中的 H 的测定具有良好的回收率（98-105%），这使其在食品新鲜度评估中具有很大的应用潜力。最初，它在洋葱组织和斑马鱼中的检测 H 变化的良好行为成功地说明了其在生物系统中定性可视化酸 pH 值变化的能力。
