Country Department of Clinical Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, PR China; Shanghai Institute of Thoracic Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, PR China.
School of Biomedical Engineering, Institute of Medical Robotics and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, PR China.
Biosens Bioelectron. 2022 Aug 15;210:114254. doi: 10.1016/j.bios.2022.114254. Epub 2022 Apr 7.
On-site screening of diabetes and precise diagnosis of diabetic complications may provide a conduit for early intervention and disease burden reduction. However, stratified metabolic analysis needs designed materials for colorimetric detection of targeted biomarkers and direct metabolic fingerprinting of the native blood. Here, an advanced dual-modal nanoplatform is constructed based on PdPtAu alloys, which serve both as the nanoenzymes in colorimetric sensing for targeted metabolite quantitation and as matrix in laser desorption/ionization mass spectrometry for untargeted metabolic fingerprinting. The platform achieved rapid glucose quantitation toward point-of-care testing of 27 participants and identified diabetic retinopathy from diabetic population with a sensitivity and specificity of 84.6%. We further assessed the generalizability of the nanoplatform for real-case applications, through the captured digital images and computing resources equipped in smartphones. The results advance the design of material-based platforms for stratified metabolic analysis and display promise to fit in the current hierarchical medical system in practice.
现场筛查糖尿病并精确诊断糖尿病并发症，可能为早期干预和减轻疾病负担提供途径。然而，分层代谢分析需要设计材料来进行针对生物标志物的比色检测，并直接对原生血液进行代谢指纹分析。在这里，构建了一种基于 PdPtAu 合金的先进双模纳米平台，它既可用作比色传感中的纳米酶，用于靶向代谢物定量，也可用作基质，用于激光解吸/电离质谱法进行非靶向代谢指纹分析。该平台针对 27 名参与者的即时护理检测实现了快速葡萄糖定量，并从糖尿病患者中以 84.6%的灵敏度和特异性识别出糖尿病性视网膜病变。我们还通过智能手机配备的捕获数字图像和计算资源，评估了纳米平台在实际应用中的通用性。研究结果推动了基于材料的分层代谢分析平台的设计，并有望在实际中符合当前的分层医疗系统。
