Kumar Ravindra, Mishra Sweta, Gwal Anil, Shanmugam Rajasubramaniam
Division of Genetic Disorders, ICMR-National Institute of Research in Tribal Health, Nagpur Road, P.O. Garha, Jabalpur, Madhya Pradesh 482003 India.
Indian J Clin Biochem. 2022 Apr;37(2):185-191. doi: 10.1007/s12291-021-00962-5. Epub 2021 Feb 8.
The aim of the study is to evaluate the stability and longevity of the paper-based screening test for the sickle cell disease in relation to different temperatures and storage time. Blood stain patterns were interpreted after spotting the blood-buffer mixture (phosphate buffer, saponin and sodium metabisulfite) on chromatographic paper (Whatman no. 3). The stability of the buffer was tested after keeping the buffer at different temperature for 24 h. Longevity of the buffer was tested post storage for various time intervals. Test indicated reproducibility with the buffer stored at 4°C. The 15% metabisulfite buffer was found to be stable up to 180 days at 4°C and showed accurate identification of all genotypes. The tests revealed 100% sensitivity and 100% specificity in identification of HbS. However, the sensitivity of differentiation between sickle cell trait (AS) with disease (SS) was found to be 97.7% with 100% specificity. The paper-based screening test may be used as a method of choice for the screening of sickle cell anemia in community-based screening programs. The low-cost, rapid, and accurate point of care testing tools offer an avenue for effective screening in developing nations.
本研究的目的是评估针对镰状细胞病的纸质筛查试验在不同温度和储存时间下的稳定性和寿命。在将血液-缓冲液混合物（磷酸盐缓冲液、皂苷和焦亚硫酸钠）点样于色谱纸（Whatman 3号滤纸）上之后，对血迹模式进行解读。在将缓冲液于不同温度下保存24小时后，测试缓冲液的稳定性。在储存后的不同时间间隔对缓冲液的寿命进行测试。测试表明，储存于4°C的缓冲液具有可重复性。发现15%的焦亚硫酸钠缓冲液在4°C下长达180天都是稳定的，并且能准确鉴定所有基因型。这些测试在鉴定血红蛋白S（HbS）方面显示出100%的敏感性和100%的特异性。然而，镰状细胞性状（AS）与疾病（SS）之间鉴别的敏感性为97.7%，特异性为100%。在社区筛查项目中，纸质筛查试验可作为镰状细胞贫血筛查的首选方法。这种低成本、快速且准确的即时检测工具为发展中国家的有效筛查提供了一条途径。
