Roth M G, Gundersen D, Patil N, Rodriguez-Boulan E
J Cell Biol. 1987 Mar;104(3):769-82. doi: 10.1083/jcb.104.3.769.
MA104.11 rhesus kidney cells express several characteristics of polarized epithelial cells, including the formation of "domes" on impermeable substrates, the establishment of a transmonolayer electrical resistance when grown on collagen gels, the polarized maturation of influenza and vesicular stomatitis viruses, and the expression of the glycoproteins of those viruses at a single surface domain. The polarized expression of the influenza virus hemagglutinin (HA) is maintained in MA104.11 cells infected with SV40-derived vectors carrying a cDNA gene for either the wild-type influenza virus HA, a truncated HA gene encoding a secreted form of HA (HAsec), or a chimeric gene encoding a hybrid protein with the external domain of the HA and the transmembrane and cytoplasmic domains of the vesicular stomatitis virus G protein (HAG). Thus, the recognition event separating glycoproteins, such as HA, destined for the apical surface from proteins, such as G, destined for the basolateral membranes involves features of the external domains of the proteins. The transmembrane and cytoplasmic domains of HA have no role in this process.
MA104.11恒河猴肾细胞表现出极化上皮细胞的几种特征，包括在不可渗透底物上形成“穹顶”、在胶原凝胶上生长时建立跨单层电阻、流感病毒和水疱性口炎病毒的极化成熟，以及这些病毒的糖蛋白在单个表面结构域的表达。流感病毒血凝素（HA）的极化表达在感染了携带野生型流感病毒HA的cDNA基因、编码分泌形式HA（HAsec）的截短HA基因或编码具有HA外部结构域与水疱性口炎病毒G蛋白跨膜和胞质结构域的杂合蛋白（HAG）的SV40衍生载体的MA104.11细胞中得以维持。因此，将注定定位于顶端表面的糖蛋白（如HA）与注定定位于基底外侧膜的蛋白（如G）区分开来的识别事件涉及蛋白质外部结构域的特征。HA的跨膜和胞质结构域在此过程中不起作用。
