在极化的猴肾细胞中，大的外部结构域足以对分泌型或嵌合型流感病毒血凝素进行正确分选。
The large external domain is sufficient for the correct sorting of secreted or chimeric influenza virus hemagglutinins in polarized monkey kidney cells.
作者信息
Roth M G, Gundersen D, Patil N, Rodriguez-Boulan E
出版信息
J Cell Biol. 1987 Mar;104(3):769-82. doi: 10.1083/jcb.104.3.769.
Abstract
MA104.11 rhesus kidney cells express several characteristics of polarized epithelial cells, including the formation of "domes" on impermeable substrates, the establishment of a transmonolayer electrical resistance when grown on collagen gels, the polarized maturation of influenza and vesicular stomatitis viruses, and the expression of the glycoproteins of those viruses at a single surface domain. The polarized expression of the influenza virus hemagglutinin (HA) is maintained in MA104.11 cells infected with SV40-derived vectors carrying a cDNA gene for either the wild-type influenza virus HA, a truncated HA gene encoding a secreted form of HA (HAsec), or a chimeric gene encoding a hybrid protein with the external domain of the HA and the transmembrane and cytoplasmic domains of the vesicular stomatitis virus G protein (HAG). Thus, the recognition event separating glycoproteins, such as HA, destined for the apical surface from proteins, such as G, destined for the basolateral membranes involves features of the external domains of the proteins. The transmembrane and cytoplasmic domains of HA have no role in this process.
摘要
MA104.11恒河猴肾细胞表现出极化上皮细胞的几种特征，包括在不可渗透底物上形成“穹顶”、在胶原凝胶上生长时建立跨单层电阻、流感病毒和水疱性口炎病毒的极化成熟，以及这些病毒的糖蛋白在单个表面结构域的表达。流感病毒血凝素（HA）的极化表达在感染了携带野生型流感病毒HA的cDNA基因、编码分泌形式HA（HAsec）的截短HA基因或编码具有HA外部结构域与水疱性口炎病毒G蛋白跨膜和胞质结构域的杂合蛋白（HAG）的SV40衍生载体的MA104.11细胞中得以维持。因此，将注定定位于顶端表面的糖蛋白（如HA）与注定定位于基底外侧膜的蛋白（如G）区分开来的识别事件涉及蛋白质外部结构域的特征。HA的跨膜和胞质结构域在此过程中不起作用。
相似文献
1
The large external domain is sufficient for the correct sorting of secreted or chimeric influenza virus hemagglutinins in polarized monkey kidney cells.在极化的猴肾细胞中，大的外部结构域足以对分泌型或嵌合型流感病毒血凝素进行正确分选。
J Cell Biol. 1987 Mar;104(3):769-82. doi: 10.1083/jcb.104.3.769.
2
Heterologous transmembrane and cytoplasmic domains direct functional chimeric influenza virus hemagglutinins into the endocytic pathway.异源跨膜和细胞质结构域将功能性嵌合流感病毒血凝素导向内吞途径。
J Cell Biol. 1986 Apr;102(4):1271-83. doi: 10.1083/jcb.102.4.1271.
3
Polarized expression of a chimeric protein in which the transmembrane and cytoplasmic domains of the influenza virus hemagglutinin have been replaced by those of the vesicular stomatitis virus G protein.一种嵌合蛋白的极化表达，其中流感病毒血凝素的跨膜和细胞质结构域已被水泡性口炎病毒G蛋白的相应结构域所取代。
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9318-22. doi: 10.1073/pnas.83.24.9318.
4
Microtubule-acting drugs lead to the nonpolarized delivery of the influenza hemagglutinin to the cell surface of polarized Madin-Darby canine kidney cells.作用于微管的药物导致流感血凝素在极化的犬肾细胞（Madin-Darby canine kidney cells）的细胞表面进行非极化递送。
J Cell Biol. 1987 Feb;104(2):231-41. doi: 10.1083/jcb.104.2.231.
5
A sorting signal for the basolateral delivery of the vesicular stomatitis virus (VSV) G protein lies in its luminal domain: analysis of the targeting of VSV G-influenza hemagglutinin chimeras.水泡性口炎病毒（VSV）G蛋白向基底外侧运输的分选信号位于其腔结构域：VSV G-流感血凝素嵌合体靶向分析。
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4112-6. doi: 10.1073/pnas.86.11.4112.
6
7
Basis for selective incorporation of glycoproteins into the influenza virus envelope.流感病毒包膜中糖蛋白选择性掺入的基础。
J Virol. 1993 Aug;67(8):4831-41. doi: 10.1128/JVI.67.8.4831-4841.1993.
8
Endocytosis of chimeric influenza virus hemagglutinin proteins that lack a cytoplasmic recognition feature for coated pits.缺乏被膜小窝胞质识别特征的嵌合流感病毒血凝素蛋白的内吞作用。
J Cell Biol. 1996 Jul;134(2):339-48. doi: 10.1083/jcb.134.2.339.
9
Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA.在感染携带克隆血凝素DNA的重组SV40病毒的细胞中，流感病毒血凝素的表达呈极化状态。
Cell. 1983 Jun;33(2):435-43. doi: 10.1016/0092-8674(83)90425-7.
10
Surface expression of viral glycoproteins is polarized in epithelial cells infected with recombinant vaccinia viral vectors.在感染重组痘苗病毒载体的上皮细胞中，病毒糖蛋白的表面表达呈极化状态。
EMBO J. 1986 Feb;5(2):237-45. doi: 10.1002/j.1460-2075.1986.tb04204.x.
引用本文的文献
1
ABCC1 is related to the protection of the distal nephron against hyperosmolality and high sodium environment: possible implications for cancer chemotherapy.ABCC1 与远曲小管在高渗和高钠环境中的保护有关：可能对癌症化疗有影响。
PLoS One. 2013 Jun 28;8(6):e68049. doi: 10.1371/journal.pone.0068049. Print 2013.
2
Dissecting rotavirus particle-raft interaction with small interfering RNAs: insights into rotavirus transit through the secretory pathway.利用小干扰RNA剖析轮状病毒颗粒与脂筏的相互作用：深入了解轮状病毒通过分泌途径的转运过程
J Virol. 2006 Apr;80(8):3935-46. doi: 10.1128/JVI.80.8.3935-3946.2006.
3
Exploitation of nucleic acid packaging signals to generate a novel influenza virus-based vector stably expressing two foreign genes.利用核酸包装信号构建一种新型的基于流感病毒的载体，该载体可稳定表达两个外源基因。
J Virol. 2003 Oct;77(19):10575-83. doi: 10.1128/jvi.77.19.10575-10583.2003.
4
Absence of direct delivery for single transmembrane apical proteins or their "Secretory" forms in polarized hepatic cells.极化肝细胞中单一跨膜顶端蛋白或其“分泌”形式不存在直接分泌。
Mol Biol Cell. 2002 Jan;13(1):225-37. doi: 10.1091/mbc.01-07-0376.
5
Persistent membrane association of activated and depalmitoylated G protein alpha subunits.活化的和去棕榈酰化的G蛋白α亚基与膜的持续结合。
Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):412-7. doi: 10.1073/pnas.96.2.412.
6
The cytoplasmic tail of rhodopsin acts as a novel apical sorting signal in polarized MDCK cells.视紫红质的细胞质尾巴在极化的MDCK细胞中作为一种新型的顶端分选信号。
J Cell Biol. 1998 Sep 7;142(5):1245-56. doi: 10.1083/jcb.142.5.1245.
7
Organization of G proteins and adenylyl cyclase at the plasma membrane.质膜上G蛋白与腺苷酸环化酶的组织形式。
Mol Biol Cell. 1997 Dec;8(12):2365-78. doi: 10.1091/mbc.8.12.2365.
8
The O-glycosylated stalk domain is required for apical sorting of neurotrophin receptors in polarized MDCK cells.O-糖基化的茎域是极化的MDCK细胞中神经营养因子受体顶端分选所必需的。
J Cell Biol. 1997 Nov 17;139(4):929-40. doi: 10.1083/jcb.139.4.929.
9
Rotavirus is released from the apical surface of cultured human intestinal cells through nonconventional vesicular transport that bypasses the Golgi apparatus.轮状病毒通过绕过高尔基体的非常规囊泡运输从培养的人肠道细胞顶端表面释放。
J Virol. 1997 Nov;71(11):8268-78. doi: 10.1128/JVI.71.11.8268-8278.1997.
10
Transmembrane domain of influenza virus neuraminidase, a type II protein, possesses an apical sorting signal in polarized MDCK cells.流感病毒神经氨酸酶的跨膜结构域，一种II型蛋白，在极化的MDCK细胞中具有顶端分选信号。
J Virol. 1996 Sep;70(9):6508-15. doi: 10.1128/JVI.70.9.6508-6515.1996.
本文引用的文献
1
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
2
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.流感病毒血凝素膜糖蛋白在3埃分辨率下的结构
Nature. 1981 Jan 29;289(5796):366-73. doi: 10.1038/289366a0.
3
Isolation and structural analysis of influenza C virion glycoproteins.丙型流感病毒粒子糖蛋白的分离与结构分析。
Virology. 1981 Sep;113(2):439-51. doi: 10.1016/0042-6822(81)90173-2.
4
Lectin-resistant mutants of polarized epithelial cells.极化上皮细胞的凝集素抗性突变体。
Mol Cell Biol. 1982 Oct;2(10):1287-94. doi: 10.1128/mcb.2.10.1287-1294.1982.
5
Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein.编码该蛋白质细胞内形式和分泌形式的流感血凝素基因的构建。
Nature. 1982 Dec 16;300(5893):598-603. doi: 10.1038/300598a0.
6
Haemagglutinin of influenza virus expressed from a cloned gene promotes membrane fusion.从克隆基因表达的流感病毒血凝素可促进膜融合。
Nature. 1982 Dec 16;300(5893):658-9. doi: 10.1038/300658a0.
7
Cell surface expression of the influenza virus hemagglutinin requires the hydrophobic carboxy-terminal sequences.流感病毒血凝素的细胞表面表达需要疏水的羧基末端序列。
Cell. 1982 Sep;30(2):649-56. doi: 10.1016/0092-8674(82)90261-6.
8
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.一种决定核质蛋白向细胞核迁移的多肽结构域。
Cell. 1982 Sep;30(2):449-58. doi: 10.1016/0092-8674(82)90242-2.
9
Intracellular transport of influenza virus hemagglutinin to the apical surface of Madin-Darby canine kidney cells.流感病毒血凝素在细胞内运输至犬肾传代细胞（Madin-Darby canine kidney cells）顶端表面的过程。
J Cell Biol. 1984 Jan;98(1):308-19. doi: 10.1083/jcb.98.1.308.
10
Glycosylation and surface expression of the influenza virus neuraminidase requires the N-terminal hydrophobic region.流感病毒神经氨酸酶的糖基化和表面表达需要N端疏水区域。
Mol Cell Biol. 1984 Jan;4(1):8-16. doi: 10.1128/mcb.4.1.8-16.1984.
Zotero 插件