Zhang Ruochong, Koh Sally Shuxian, Teo Mark Ju Teng, Bi Renzhe, Zhang Shuyan, Dev Kapil, Urano Daisuke, Dinish U S, Olivo Malini
Translational Biophotonic Laboratory, Institute of Bioengineering and Bioimaging, ASTAR, Singapore, Singapore.
Temasek Life Sciences Laboratory, Singapore, Singapore.
Front Plant Sci. 2022 Apr 8;13:822634. doi: 10.3389/fpls.2022.822634. eCollection 2022.
Fluorescence imaging has shown great potential in non-invasive plant monitoring and analysis. However, current systems have several limitations, such as bulky size, high cost, contact measurement, and lack of multifunctionality, which may hinder its applications in a wide range of settings including indoor vertical farming. Herein, we developed a compact handheld fluorescence imager enabling multipurpose plant phenotyping, such as continuous photosynthetic activity monitoring and non-destructive anthocyanin quantification. The compact imager comprises of pulse-amplitude-modulated multi-color light emitting diodes (LEDs), optimized light illumination and collection, dedicated driver circuit board, miniaturized charge-coupled device camera, and associated image analytics. Experiments conducted in drought stressed lettuce proved that the novel imager could quantitatively evaluate the plant stress by the non-invasive measurement of photosynthetic activity efficiency. Moreover, a non-invasive and fast quantification of anthocyanins in green and red Batavia lettuce leaves had excellent correlation (>84%) with conventional destructive biochemical analysis. Preliminary experimental results emphasize the high throughput monitoring capability and multifunctionality of our novel handheld fluorescence imager, indicating its tremendous potential in modern agriculture.
荧光成像在植物非侵入式监测与分析方面展现出了巨大潜力。然而，当前的系统存在若干局限性，如体积庞大、成本高昂、需接触式测量以及缺乏多功能性等，这些可能会阻碍其在包括室内垂直种植在内的广泛场景中的应用。在此，我们开发了一种紧凑的手持式荧光成像仪，可实现多用途植物表型分析，如连续监测光合活性和对花青素进行无损定量分析。该紧凑成像仪由脉冲幅度调制多色发光二极管（LED）、优化的光照明与采集装置、专用驱动电路板、小型化电荷耦合器件相机以及相关图像分析软件组成。在干旱胁迫的生菜上进行的实验证明，这种新型成像仪能够通过非侵入式测量光合活性效率来定量评估植物胁迫。此外，对绿色和红色巴达维亚生菜叶片中的花青素进行非侵入式快速定量分析，其结果与传统破坏性生化分析具有极好的相关性（>84%）。初步实验结果凸显了我们新型手持式荧光成像仪的高通量监测能力和多功能性，表明其在现代农业中具有巨大潜力。
