School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China.
Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan 646000, China.
STAR Protoc. 2022 Apr 11;3(2):101286. doi: 10.1016/j.xpro.2022.101286. eCollection 2022 Jun 17.
phototransduction is a well-established model for characterizing biological processes, such as calcium signaling. Here, we present a protocol for electroretinogram (ERG) recording, an physiological assay extensively used in phototransduction research to measure light-evoked field potential responses of photoreceptors and neurons in the lamina neuropil. We describe fly preparation and electrode placement, followed by pretest setup, ERG recording, and data analysis. This protocol enables assessment of photoreceptor performance and visual synaptic transmission between photoreceptors and downstream lamina neurons. For complete details on the use and execution of this protocol, please refer to Han et al. (2006), Hu et al. (2012), and Wu et al. (2021).
光转导是一种成熟的生物学过程研究模型，例如钙信号。在这里，我们介绍了视网膜电图（ERG）记录的方案，这是一种在光转导研究中广泛使用的生理测定方法，用于测量光诱发的光感受器和神经节细胞层神经元的场电位反应。我们描述了蝇的准备和电极放置，然后是预测试设置、ERG 记录和数据分析。该方案可用于评估光感受器的性能和光感受器与下游神经节细胞层之间的视觉突触传递。有关此方案使用和执行的完整详细信息，请参阅 Han 等人（2006 年）、Hu 等人（2012 年）和 Wu 等人（2021 年）。
