Bissell D M, Arenson D M, Maher J J, Roll F J
J Clin Invest. 1987 Mar;79(3):801-12. doi: 10.1172/JCI112887.
The subendothelial space of normal rat liver contains the constituent proteins of a basal lamina, as judged by immunohistochemical study of tissue sections. However, it is unknown whether these proteins constitute a complex with effects on hepatocellular function. We have examined this question, using normal rat hepatocytes cultured on substrata of matrix proteins as a model of the interaction between cells and basal lamina in vivo. In cultures on a type I collagen substratum, albumin secretion decreased progressively after 2 d. By contrast, when cells were cultured on a laminin-rich gel matrix, albumin secretion was stable for at least 3 wk; other functions and ultrastructural morphology were similarly maintained. None of the individual matrix proteins effectively substituted for the gel matrix, suggesting that full support of hepatocellular function requires a complex of matrix proteins. We speculate that a cause of hepatocellular dysfunction in acute inflammation is disruption of this matrix and alteration of its interaction with the hepatocyte plasma membrane.
通过对组织切片的免疫组织化学研究判断，正常大鼠肝脏的内皮下间隙含有基膜的组成蛋白。然而，这些蛋白是否构成对肝细胞功能有影响的复合物尚不清楚。我们使用在基质蛋白底物上培养的正常大鼠肝细胞作为体内细胞与基膜相互作用的模型，研究了这个问题。在I型胶原底物上培养时，白蛋白分泌在2天后逐渐减少。相比之下，当细胞在富含层粘连蛋白的凝胶基质上培养时，白蛋白分泌至少3周保持稳定；其他功能和超微结构形态也同样得以维持。没有一种单一的基质蛋白能有效替代凝胶基质，这表明肝细胞功能的充分维持需要基质蛋白复合物。我们推测，急性炎症中肝细胞功能障碍的一个原因是这种基质的破坏及其与肝细胞质膜相互作用的改变。
