Dunn J C, Yarmush M L, Koebe H G, Tompkins R G
Department of Surgery, Massachusetts General Hospital, Boston 02114.
FASEB J. 1989 Feb;3(2):174-7. doi: 10.1096/fasebj.3.2.2914628.
Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and a physiological rate of albumin secretion for at least 42 days. Hepatocytes cultured on a single layer of collagen gel essentially ceased albumin secretion within 1 wk but could recover function with the overlay of a second layer of collagen gel. This culture configuration more closely mimics the hepatocytes' in vivo environment and provides a simple method for their long-term maintenance.
在胶原三明治系统中培养的成年大鼠肝细胞可保持正常形态，并在至少42天内维持生理水平的白蛋白分泌率。在单层胶原凝胶上培养的肝细胞在1周内基本停止白蛋白分泌，但在覆盖第二层胶原凝胶后可恢复功能。这种培养方式更接近肝细胞的体内环境，并为其长期维持提供了一种简单的方法。
