Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, The Volcani Institute, Rishon LeZion, 7505101, Israel.
Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, The Volcani Institute, Rishon LeZion, 7505101, Israel.
Environ Microbiol. 2022 Aug;24(8):3369-3389. doi: 10.1111/1462-2920.16016. Epub 2022 May 2.
Fungi associated with cypress bark beetles are practically unknown in the Eastern Mediterranean. Our study focused on the fungi associated with the body parts and galleries of two indigenous cypress bark beetles, Phloeosinus armatus and P. bicolor, sampled from Cupressus sempervirens trees in different regions in Israel. Arbitrarily primed PCR, performed on genomic DNA of 302 isolates, clustered the fungal population into five distinct groups. Multilocus phylogeny, split-network analyses and morphological characterization identified the isolates as Geosmithia omnicola, Geosmithia langdonii, Geosmithia sp. 708b, Geosmithia cupressina sp. nov. CBS147103 and Talaromyces cupressi sp. nov. CBS147104. Of these fungal isolates, G. cupressina and T. cupressi are newly described, and their morphological features and phylogenetic designations are presented. Inoculation of intact cypress saplings in an outdoor net-house revealed that only the representative isolate T. cupressi sp. nov. CBS147104 causes 100% disease incidence, whereas Geosmithia spp. isolates are not pathogenic. A number of these fungi were isolated from parasitoids that emerged from branch and stem sections colonized by P. armatus. This study suggests a long and stable association between Phloeosinus and Geosmithia species, and a possible role for additional associated fungal species as pathogens or endophytes of C. sempervirens trees in Israel.
在地中海东部，与柏木树皮甲虫相关的真菌实际上是未知的。我们的研究集中在与两种本地柏木树皮甲虫，即 Phloeosinus armatus 和 P. bicolor 的身体部位和坑道相关的真菌上，这些甲虫是从以色列不同地区的 Cupressus sempervirens 树上采集的。对 302 个分离株的基因组 DNA 进行任意引物 PCR，将真菌种群聚类为五个不同的群体。多基因系统发育、分裂网络分析和形态特征鉴定将分离株鉴定为 Geosmithia omnicola、Geosmithia langdonii、Geosmithia sp. 708b、Geosmithia cupressina sp. nov. CBS147103 和 Talaromyces cupressi sp. nov. CBS147104。在这些真菌分离物中，G. cupressina 和 T. cupressi 是新描述的，它们的形态特征和系统发育指定被提出。在户外网室内对完整的柏树苗进行接种表明，只有代表性分离株 T. cupressi sp. nov. CBS147104 引起 100%的发病率，而 Geosmithia spp. 分离物则没有致病性。一些真菌是从 Phloeosinus 寄生蜂从被 P. armatus 定植的树枝和茎段中出现的寄生蜂中分离出来的。本研究表明 Phloeosinus 和 Geosmithia 物种之间存在长期而稳定的联系，并且其他相关真菌物种可能作为病原体或内生菌在以色列的 C. sempervirens 树上发挥作用。
