Confraria Ana, Muñoz-Gasca Aitor, Ferreira Liliana, Baena-González Elena, Cubas Pilar
Instituto Gulbenkian de Ciência, Oeiras, Portugal.
GREEN-IT Bioresources for Sustainability, ITQB NOVA, Oeiras, Portugal.
Methods Mol Biol. 2022;2494:47-59. doi: 10.1007/978-1-0716-2297-1_5.
Shoot branching is an important trait that depends on the activity of axillary meristems and buds and their outgrowth into branches. It is remarkably plastic, being influenced by a number of external cues, such as light, temperature, soil nutrients, and mechanical manipulation. These are transduced into an internal hormone signaling network where auxin, cytokinins, and strigolactones play leading regulatory roles. Recently, sugars have also emerged as important signals promoting bud activation. These signals are in part integrated by the bud-specific growth repressor BRANCHED1 (BRC1).To understand how shoot branching is affected by particular growth conditions or in specific plant lines, it is necessary to count the number of branches and/or quantify other branch-related parameters. Here we describe how to perform such quantifications in Arabidopsis and in tomato.
茎枝分枝是一个重要的性状，它取决于腋生分生组织和芽的活性以及它们生长为枝条的过程。它具有显著的可塑性，受到许多外部信号的影响，如光照、温度、土壤养分和机械处理。这些外部信号被转化为一个内部激素信号网络，其中生长素、细胞分裂素和独脚金内酯发挥着主要的调节作用。最近，糖类也已成为促进芽激活的重要信号。这些信号部分由芽特异性生长抑制因子BRANCHED1（BRC1）整合。为了了解特定生长条件或特定植物品系如何影响茎枝分枝，有必要对枝条数量进行计数和/或对其他与分枝相关的参数进行量化。在这里，我们描述了如何在拟南芥和番茄中进行此类量化。
