蔗糖通过细胞分裂素促进茎分枝。
Sucrose promotes stem branching through cytokinin.
机构信息
Department of Postharvest Science, The Volcani Center, ARO, Rishon LeZion, Israel.
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel.
出版信息
Plant Physiol. 2021 Apr 23;185(4):1708-1721. doi: 10.1093/plphys/kiab003.
Shoot branching is an important aspect of plant architecture because it substantially affects plant biology and agricultural performance. Sugars play an important role in the induction of shoot branching in several species, including potato (Solanum tuberosum L.). However, the mechanism by which sugars affect shoot branching remains mostly unknown. In the present study, we addressed this question using sugar-mediated induction of bud outgrowth in potato stems under etiolated conditions. Our results indicate that sucrose feeding to detached stems promotes the accumulation of cytokinin (CK), as well as the expression of vacuolar invertase (VInv), an enzyme that contributes to sugar sink strength. These effects of sucrose were suppressed by CK synthesis and perception inhibitors, while CK supplied to detached stems induced bud outgrowth and VInv activity in the absence of sucrose. CK-induced bud outgrowth was suppressed in vinv mutants, which we generated by genome editing. Altogether, our results identify a branching-promoting module, and suggest that sugar-induced lateral bud outgrowth is in part promoted by the induction of CK-mediated VInv activity.
分枝是植物结构的一个重要方面，因为它极大地影响了植物的生物学和农业表现。糖在包括马铃薯（Solanum tuberosum L.）在内的几个物种中诱导分枝方面发挥着重要作用。然而，糖影响分枝的机制在很大程度上仍然未知。在本研究中，我们在黑暗条件下使用糖介导的马铃薯茎芽伸长诱导来解决这个问题。我们的结果表明，向离体茎中添加蔗糖可促进细胞分裂素（CK）的积累，以及液泡转化酶（VInv）的表达，后者有助于糖汇强度。蔗糖的这些作用被 CK 合成和感知抑制剂所抑制，而 CK 供应到离体茎中可在没有蔗糖的情况下诱导芽伸长和 VInv 活性。通过基因组编辑，我们生成了 vinv 突变体，其 CK 诱导的芽伸长受到抑制。总的来说，我们的结果确定了一个促进分枝的模块，并表明糖诱导的侧芽伸长部分是通过诱导 CK 介导的 VInv 活性来促进的。
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