Lai Andrew, Palma Carlos, Salas Alexis, Carrion Flavio, Salomon Carlos
Exosome Biology Laboratory, University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital, The University of Queensland, Brisbane, QLD, Australia.
Department of Pharmacology, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
Methods Mol Biol. 2022;2504:79-89. doi: 10.1007/978-1-0716-2341-1_6.
Extracellular vesicles (EVs) carry a wide range of molecules, such as proteins, RNAs, and DNA. EVs are secreted from a wide range of cells, including placental cells. Interestingly, EVs secreted from placental cells have been identified in maternal circulation as early as 6 weeks of gestation, and their concentration increases with the gestational age. While there is growing interest in elucidating the role of exosomes during normal and complicated pregnancies, progress in the field has been delayed because of the inability to quantify placental EVs from the maternal circulation. Recent reports have demonstrated the presence of placental-type alkaline phosphatase (PLAP) EVs only in the blood of pregnant women, indicating that PLAP is a marker to identify EVs secreted from the placenta. Therefore, here we describe a workflow to quantify placental EVs from maternal circulation using a targeted proteomics approach based on selecting specific peptides identified in the PLAP protein.
细胞外囊泡（EVs）携带多种分子，如蛋白质、RNA和DNA。EVs由多种细胞分泌，包括胎盘细胞。有趣的是，早在妊娠6周时，母体循环中就已鉴定出胎盘细胞分泌的EVs，并且其浓度随孕周增加而升高。虽然人们越来越关注阐明外泌体在正常和复杂妊娠中的作用，但由于无法从母体循环中定量胎盘EVs，该领域的进展一直滞后。最近的报告表明，仅在孕妇血液中存在胎盘型碱性磷酸酶（PLAP）EVs，这表明PLAP是识别胎盘分泌的EVs的标志物。因此，在此我们描述了一种工作流程，该流程基于选择在PLAP蛋白中鉴定出的特定肽段，采用靶向蛋白质组学方法从母体循环中定量胎盘EVs。
