Wu Qiang, Ke Min-Xia, Yang Huang-Tian
CAS Key Laboratory of Tissue Microenvironment and Tumor, Laboratory of Molecular Cardiology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences (CAS), CAS, Shanghai, China.
Translational Medical Center for Stem Cell Therapy and Institute for Heart Failure and Regenerative Medicine, Shanghai East Hospital, Tongji University School of Medicine and Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China.
Methods Mol Biol. 2022;2504:91-100. doi: 10.1007/978-1-0716-2341-1_7.
Extracellular vesicles (EVs) secreted by human pluripotent stem cells-derived cardiovascular progenitor cells (hPSC-CVPCs) can improve repair of infarcted hearts in mouse and nonhuman primate myocardial infarction models. To fully achieve their values, it is essential to establish an efficient method for the isolation of EVs from hPSC-CVPCs. Here we describe the protocols for efficient isolation and characterization of EVs from the conditioned medium of hPSC-CVPCs.
人多能干细胞衍生的心血管祖细胞（hPSC-CVPCs）分泌的细胞外囊泡（EVs）可改善小鼠和非人类灵长类动物心肌梗死模型中梗死心脏的修复。为了充分实现其价值，建立一种从hPSC-CVPCs中高效分离EVs的方法至关重要。在此，我们描述了从hPSC-CVPCs条件培养基中高效分离和鉴定EVs的方案。
