Zhang Peng, Yao Lejing, Shan Guoping, Chen Yuanyuan
Department of Radiology Physics, Zhejiang Key Laboratory of Radiation Oncology, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, China.
Department of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China.
Int J Radiat Biol. 2022 Apr 27;98(11):1645-1654. doi: 10.1080/09553002.2022.2069298. Print 2022.
A small animal radiation research platform (SARRP) equipped with a miniature beam system, an image-guided positioning system, and a dose planning system was used to develop and evaluate a mouse model of radiation-induced temporomandibular damage.
Left jaw disks of adult male C57BL/6 mice and C3H mice were targeted using the SARRP for image-guided irradiation. The total radiation dose was 75 Gy. Experiment 1 (Scoping study): Mice in the C57BL/6 mouse test and control groups were sacrificed at 1, 3, 6, 9, 12, 15, and 18 weeks after irradiation, whereas mice in the C3H test and control groups were sacrificed at 1, 3, 6, 9, and 12 weeks after irradiation. Experiment 2 (Full -scale validation study): Mice in the C57BL/6 mouse test and control groups were sacrificed at 1, 3 and 6 weeks after irradiation. Histopathological analysis of the temporomandibular skeletal muscle in each group was performed using hematoxylin and eosin (H&E) and Masson staining; the temporal mandibular bone was examined through H&E staining.
SARRP delivered the rated dose to the temporomandibular joints of C57BL/6 and C3H mice. C3H and C57BL/6 mice in the test group showed different degrees of osteocytic necrosis and osteoporosis at different time points. H&E staining of skeletal muscle tissue showed slight fibrosis in the C57BL/6 test at 3 and 6 weeks time point.
We established a model of radiation-induced damage in the temporomandibular joint of C57BL/6 mice and demonstrated that the observed physiological and histological changes correspond to radiation damage observed in humans. Furthermore, the SARRP can deliver precise radiation doses.
使用配备微型束流系统、图像引导定位系统和剂量规划系统的小动物辐射研究平台（SARRP）来开发和评估辐射诱导的颞下颌损伤小鼠模型。
使用SARRP对成年雄性C57BL/6小鼠和C3H小鼠的左颌盘进行图像引导照射。总辐射剂量为75 Gy。实验1（范围研究）：C57BL/6小鼠试验组和对照组的小鼠在照射后1、3、6、9、12、15和18周处死，而C3H试验组和对照组的小鼠在照射后1、3、6、9和12周处死。实验2（全面验证研究）：C57BL/6小鼠试验组和对照组的小鼠在照射后1、3和6周处死。对每组颞下颌骨骼肌进行苏木精-伊红（H&E）染色和Masson染色的组织病理学分析；通过H&E染色检查颞下颌骨。
SARRP向C57BL/6和C3H小鼠的颞下颌关节输送了额定剂量。试验组中的C3H和C57BL/6小鼠在不同时间点表现出不同程度的骨细胞坏死和骨质疏松。骨骼肌组织的H&E染色显示，在3周和6周时间点，C57BL/6试验组有轻微纤维化。
我们建立了C57BL/6小鼠颞下颌关节辐射诱导损伤的模型，并证明观察到的生理和组织学变化与人类观察到的辐射损伤相对应。此外，SARRP可以输送精确的辐射剂量。
