荧光标记如何影响由引发链介导的DNA链置换反应的动力学。
How fluorescent labels affect the kinetics of the toehold-mediated DNA strand displacement reaction.
作者信息
Li Chengxu, Li Zhigang, Han Wenjie, Yin Xue, Liu Xiaoyu, Xiao Shiyan, Liang Haojun
机构信息
Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.
出版信息
Chem Commun (Camb). 2022 May 12;58(39):5849-5852. doi: 10.1039/d2cc01072k.
End modification of the toehold domain or near to it using fluorophore dyes or quenchers can significantly modulate the kinetics of the toehold-mediated strand displacement reaction (TMSDR) by almost two orders of magnitude. The labels at the end of the signal strand impede the TMSDR, while those at the end of the toehold domain of the substrate strand accelerate the TMSDR kinetics.
使用荧光团染料或猝灭剂对引发链结构域末端或其附近进行修饰，可显著调节引发链介导的链置换反应（TMSDR）的动力学，幅度可达近两个数量级。信号链末端的标记会阻碍TMSDR，而底物链引发链结构域末端的标记则会加速TMSDR动力学。
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