Liu Qi, Mao Yufeng, Liao Xiaoping, Luo Jiahao, Ma Hongwu, Jiang Wenxia
Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Apr 25;38(4):1408-1420. doi: 10.13345/j.cjb.210797.
Ergothioneine is a multifunctional physiological cytoprotector, with broad application in foods, beverage, medicine, cosmetics and so on. Biosynthesis is an increasingly favored method in the production of ergothioneine. This paper summarizes the new progress in the identification of key pathways, the mining of key enzymes, and the development of natural edible mushroom species and high-yield engineering strains for ergothioneine biosynthesis in recent years. Through this review, we aim to reveal the molecular mechanism of ergothioneine biosynthesis and then employ the methods of fermentation engineering, metabolic engineering, and synthetic biology to greatly increase the yield of ergothioneine.
麦角硫因是一种多功能的生理性细胞保护剂，在食品、饮料、医药、化妆品等领域有广泛应用。生物合成是近年来麦角硫因生产中越来越受青睐的方法。本文综述了近年来在麦角硫因生物合成关键途径的鉴定、关键酶的挖掘以及天然食用菌品种和高产工程菌株开发方面的新进展。通过本综述，我们旨在揭示麦角硫因生物合成的分子机制，进而运用发酵工程、代谢工程和合成生物学方法大幅提高麦角硫因的产量。
