利用厌氧细菌基因在[具体生物]中异源高产麦角硫因
Heterologous and High Production of Ergothioneine in by Using Genes from Anaerobic Bacteria.
作者信息
Liu Zhe, Xiao Fengxu, Zhang Yupeng, Lu Jiawei, Li Youran, Shi Guiyang
机构信息
School of Biotechnology, Key Laboratory of Carbohydrate Chemistry, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.
National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
出版信息
Metabolites. 2025 Jan 12;15(1):45. doi: 10.3390/metabo15010045.
PURPOSE
This study aimed to utilize genetically engineered for the production of ergothioneine (EGT). Given the value of EGT and the application of in enzyme preparation production, we cloned the key enzymes (EanA and EanB) from . Through gene alignment, new ergothioneine synthase genes (EanAN and EanBN) were identified and then expressed in to construct strains. Additionally, we investigated the factors influencing the yield of EGT and made a comparison with .
METHODS
The relevant genes were cloned and transferred into . Fermentation experiments were conducted under different conditions for yield analysis, and the stability of this bacterium was also evaluated simultaneously.
RESULTS
The constructed strains were capable of producing EGT. Specifically, the yield of the EanANBN strain reached (643.8 ± 135) mg/L, and its stability was suitable for continuous production.
CONCLUSIONS
Genetically engineered demonstrates potential in the industrial-scale production of EGT. Compared with , it has advantages, thus opening up new possibilities for the application and market supply of EGT.
目的
本研究旨在利用基因工程技术生产麦角硫因（EGT）。鉴于EGT的价值及其在酶制剂生产中的应用，我们从[具体来源]克隆了关键酶（EanA和EanB）。通过基因比对，鉴定出新型麦角硫因合酶基因（EanAN和EanBN），然后在[具体宿主]中表达以构建菌株。此外，我们研究了影响EGT产量的因素，并与[对比对象]进行了比较。
方法
克隆相关基因并将其转入[具体宿主]。在不同条件下进行发酵实验以分析产量，同时评估该细菌的稳定性。
结果
构建的菌株能够生产EGT。具体而言，EanANBN菌株的产量达到（643.8±135）mg/L，其稳定性适合连续生产。
结论
基因工程[具体宿主]在EGT的工业规模生产中显示出潜力。与[对比对象]相比，它具有优势，从而为EGT的应用和市场供应开辟了新的可能性。
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