Laboratorio de Investigaciones en Parasitología Tropical, Universidad del Tolima, Ibagué, Colombia.
Departamento de Parasitología, Instituto de Ciencias Biológicas, Universidad Federal de Minas Gerais, Belo Horizonte, Brasil.
Biomedica. 2022 Mar 1;42(1):136-146. doi: 10.7705/biomedica.6251.
Introduction: Toxoplasma gondii is a parasite with great zoonotic potential. It can infect a broad range of warm-blooded hosts (including livestock) and causes significant losses in the industry. In humans, it has been described as a pathogen in immunosuppressed people, it affects the fetus development in congenital infections, and is associated with various behavioral disorders in healthy people. Humans can acquire T. gondii by consuming undercooked, contaminated meat. Objective: To determine T. gondii positivity (currently unknown) in meat for human consumption (i.e., beef, chicken, and pork) in the city of Ibague, Colombia. Materials and methods: We used conventional nested PCR and the T. gondii B1 gene sequence as amplification target. We collected samples of meat (N=186) sold in the urban area of Ibagué (62 beef, 62 chicken, and 62 pork samples) and determined the T. gondii positivity percentage for each type of meat. Results: The study found an average of 18.8% positivity for all meat samples, pork having the highest percentage (22.5%; 14/62), followed by beef (19.3%; 12/62) and chicken (14.5%; 9/62). The best-amplified products were sequenced by macrogen and aligned with the B1 gene sequences in GenBank, thereby confirming their identity. Conclusions: This is the first study of T. gondii prevalence in meat for human consumption carried out in the city of Ibagué and the department of Tolima. All three types of meat sampled represent a risk for local human infection
刚地弓形虫是一种具有巨大人畜共患病潜力的寄生虫。它可以感染广泛的温血宿主（包括家畜），并在产业中造成重大损失。在人类中，它被描述为免疫抑制人群中的病原体，它会影响先天性感染的胎儿发育，并与健康人群中的各种行为障碍有关。人类可以通过食用未煮熟、受污染的肉来感染刚地弓形虫。目的：确定哥伦比亚伊瓦格市人食用肉（即牛肉、鸡肉和猪肉）中的刚地弓形虫阳性率（目前未知）。材料和方法：我们使用常规巢式 PCR 和刚地弓形虫 B1 基因序列作为扩增靶标。我们收集了伊瓦格市区出售的肉类样本（N=186），包括 62 份牛肉、62 份鸡肉和 62 份猪肉，并确定了每种肉类的刚地弓形虫阳性率。结果：研究发现，所有肉类样本的平均阳性率为 18.8%，猪肉的阳性率最高（22.5%；14/62），其次是牛肉（19.3%；12/62）和鸡肉（14.5%；9/62）。最佳扩增产物由 macrogen 测序，并与 GenBank 中的 B1 基因序列进行比对，从而确认其身份。结论：这是伊瓦格市和托利马省首次进行的人食用肉类中刚地弓形虫流行率研究。所有三种采样的肉类都代表了当地人类感染的风险。
