State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
Infect Genet Evol. 2017 Nov;55:172-174. doi: 10.1016/j.meegid.2017.08.022. Epub 2017 Aug 24.
Toxoplasma gondii can infect nearly all warm-blooded animals including birds. However, limited information on the molecular epidemiology and genotypes of T. gondii infecting poultry is available in China. Therefore, the present study characterized T. gondii genotypes in poultry meat in eastern China. During August 2015 and September 2016, muscle tissue samples collecting from 414 poultries (257 chickens, 115 ducks and 42 geese) in Shandong provinces were used to detect the T. gondii B1 gene by a semi-nested PCR, and the positive samples were genotyped at 10 nuclear loci (i.e., SAG1, alternative SAG2, 5'- and 3'-SAG2, SAG3, L358, BTUB, c22-8, GRA6, c29-2, PK1) and an apicoplast locus Apico by multi-locus PCR-RFLP technology. Thirty-two (7.37%) samples from 414 poultry meat were T. gondii B1 gene positive. Chicken had the highest T. gondii prevalence (8.17%), followed by ducks (7.83%) and geese (4.76%). Furthermore, only one genotype (ToxoDB#9) was identified. This is the first genetic characterization of T. gondii isolates from poultry meat in Shandong province, eastern China and also the first report of genotype ToxoDB#9 was found in poultry in China, which provide basic data for the surveillance and control of T. gondii infection in poultry, other animals and humans.
刚地弓形虫可感染包括鸟类在内的几乎所有温血动物。然而，目前在中国有关感染家禽的弓形虫分子流行病学和基因型的信息有限。因此，本研究对中国东部地区禽肉中的弓形虫基因型进行了特征描述。2015 年 8 月至 2016 年 9 月，从山东省的 414 只家禽（257 只鸡、115 只鸭和 42 只鹅）中采集肌肉组织样本，通过半巢式 PCR 检测弓形虫 B1 基因，阳性样本采用多基因座 PCR-RFLP 技术在 10 个核基因座（即 SAG1、替代 SAG2、5'-和 3'-SAG2、SAG3、L358、BTUB、c22-8、GRA6、c29-2、PK1）和一个质体基因座 Apico 上进行基因分型。在 414 份禽肉样本中，有 32 份（7.37%）为弓形虫 B1 基因阳性。鸡的弓形虫感染率最高（8.17%），其次是鸭（7.83%）和鹅（4.76%）。此外，仅鉴定出一种基因型（ToxoDB#9）。这是中国东部山东省首次对禽肉中弓形虫分离株进行遗传特征描述，也是首次在中国报道禽类中存在基因型 ToxoDB#9，为弓形虫感染家禽、其他动物和人类的监测和控制提供了基础数据。
