Yousefvand Amin, Mirhosseini Seyed Ali, Ghorbani Masoud, Mohammadzadeh Tahereh, Moghaddam Mehrdad Moosazadeh, Mohammadyari Shirin
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiatallah University of Medical Sciences, Tehran, Iran.
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
J Verbrauch Lebensm. 2021;16(2):117-127. doi: 10.1007/s00003-020-01306-w. Epub 2021 Jan 2.
Toxoplasmosis is one of the most important zoonotic diseases with serious health risks for humans, especially for immunodeficient patients, and can lead to abortion in pregnant women worldwide. The oral uptake of sporulated oocysts and/or consumption of undercooked/raw meat of animals infected with can infect other animals and humans Heart, liver, and meat tissues of 150 sheep and 150 goats from a slaughterhouse in Ahvaz, Iran, were collected during autumn 2018 and analyzed via polymerase chain reaction (PCR) to detect parasitic DNA in the animal tissues. Moreover, antibodies against of 150 sera samples were detected as the targets by in-house enzyme-linked immunosorbent assay (in-house ELISA). A total of 26 (17.3%), 33 (22%), and 48 (32%) of liver, meat, and heart samples in sheep, and a total of 24 (16%), 26 (17.3%), and 36 (24%) of liver, meat, and heart samples in goats, respectively, showed positive PCR results. Besides, the ELISA evaluation of sera samples from 150 sheep and 150 goats resulted in 26 (13.3%) and 16 (10.6%) positive cases, respectively. A significant difference was also found between PCR-positive heart samples and ELISA-positive sera samples of both animal species (p < 0.05), but no significant difference existed between PCR-positive liver samples and ELISA-positive sera samples of both species (p > 0.05). The results of this study confirm the presence of in sheep and goats' consumable organs, highlighting the need to avoid consuming raw or uncooked organs of these animal species to prevent human infection with .
弓形虫病是最重要的人畜共患病之一，对人类尤其是免疫功能低下的患者具有严重的健康风险，并且可导致全球孕妇流产。经孢子化的卵囊经口摄入和/或食用感染了弓形虫的动物的未煮熟/生肉可感染其他动物和人类。2018年秋季，从伊朗阿瓦士的一家屠宰场采集了150只绵羊和150只山羊的心脏、肝脏和肉组织，并通过聚合酶链反应（PCR）进行分析，以检测动物组织中的寄生虫DNA。此外，通过自制酶联免疫吸附测定法（自制ELISA）检测150份血清样本中的抗弓形虫抗体作为目标。绵羊肝脏、肉和心脏样本中分别有26份（17.3%）、33份（22%）和48份（32%），山羊肝脏、肉和心脏样本中分别有24份（16%）、26份（17.3%）和36份（24%）的PCR结果呈阳性。此外，对150只绵羊和150只山羊的血清样本进行ELISA评估，结果分别有26份（13.3%）和16份（10.6%）呈阳性。在两种动物的PCR阳性心脏样本和ELISA阳性血清样本之间也发现了显著差异（p<0.05），但两种动物的PCR阳性肝脏样本和ELISA阳性血清样本之间没有显著差异（p>0.05）。本研究结果证实了绵羊和山羊可食用器官中存在弓形虫，强调有必要避免食用这些动物物种的生的或未煮熟的器官，以防止人类感染弓形虫。
