Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
Department of Biology, Duke University, Durham, North Carolina, United States of America.
PLoS One. 2022 Apr 26;17(4):e0265817. doi: 10.1371/journal.pone.0265817. eCollection 2022.
Phaseolus vulgaris, an essential food and source of protein, is cultivated across the world. This study was carried out to investigate the diversity and population structure of 34 P. vulgaris landrace accessions collected from the Azad Jammu and Kashmir (AJ&K) regions of Pakistan. The samples were analyzed both morphologically and using genetic variation identified through RNA sequencing. Our results indicated that most genetic variation occurs among local accessions, with little genetic variation occurring between geographical regions. In addition, the accessions fell into two major genetic groups. Morphological analysis revealed that these two genetic groups differ in a number of quantitative traits, including seed length, seed width, and seed weight. One accession, DUD-11, appears to be a mixture of the two major groups genetically as well as morphologically. Among the other accessions, DUD-8, RWK-2, and NGD-1 depicted particularly high seed weight along with higher seed length, seed width, and seed yield per plant. We suggest focusing on these accessions in future breeding programs. More generally, our results provide baseline data that will be useful for crop improvement and effective cultivation practices in Pakistan.
菜豆是一种重要的食物和蛋白质来源，在世界各地都有种植。本研究旨在调查从巴基斯坦阿扎德克什米尔（AJ&K）地区收集的 34 个菜豆地方品种的多样性和种群结构。这些样本既通过形态学分析，也通过 RNA 测序确定的遗传变异进行了分析。我们的结果表明，大多数遗传变异发生在当地品种之间，地理区域之间的遗传变异很小。此外，这些品种分为两个主要的遗传群体。形态学分析表明，这两个遗传群体在一些数量性状上存在差异，包括种子长度、种子宽度和种子重量。一个品种 DUD-11 在遗传和形态上似乎是这两个主要群体的混合物。在其他品种中，DUD-8、RWK-2 和 NGD-1 的种子重量特别高，同时种子长度、种子宽度和每株植物的种子产量也较高。我们建议在未来的育种计划中关注这些品种。更普遍地说，我们的结果提供了基础数据，这将有助于在巴基斯坦进行作物改良和有效种植实践。
