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利用简单序列重复标记分析巴基斯坦普通菜豆(L.)生态型的遗传多样性

Genetic diversity of common bean ( L.) ecotypes from Pakistan using Simple Sequence Repeats.

作者信息

Jannat Sammyia, Hussain Shah Asad, Ul Hassan Mahmood, Sher Ahmad, Fiaz Sajid, Elesawy Basem H, Ahmed Ismail Khadiga, El Askary Ahmad, Gharib Amal F, Qayyum Abdul

机构信息

Department of Biotechnology, University of Kotli, Azad Jammu and Kashmir, Pakistan.

Department of Plant Breeding and Genetics, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, Pakistan.

出版信息

Saudi J Biol Sci. 2022 Jun;29(6):103300. doi: 10.1016/j.sjbs.2022.103300. Epub 2022 Apr 22.

Abstract

Common bean ( L.) is a legume crop grown all over the world and is a very important food of mountain population of Pakistan for protein intake. The Western Himalayan Mountains are rich in biodiversity including unexplored landraces of the common bean crop. Unfortunately, very little attention has been given to this valuable crop in Pakistan, and it is being exported, majorly from Ethiopia, to meet the country's requirements. The exploitation, utilization, preservation and multiplication of existing germplasm within the area are very important for sustainable production of the crop and enhancing the nutrition value for the local community in mountain regions. A research study was conducted for evaluation of biological diversity of common bean landraces from Azad Kashmir and Northern areas of Pakistan using morpho-physiological and molecular markers. Thirty-five common bean ecotypes along with one check variety were collected from different altitudes of Azad Kashmir and Northern Pakistan and screened for biological diversity. Morphological characterization revealed high genetic diversity in parameters including stem anthocyanin, growth type, days to flowering, pods/plant and 100 seeds weight. Genomic characterization using SSR markers, for allelic diversity evaluation among germplasm, also provided diverse profile with 83.3% polymorphism in banding pattern. The bulk of gene pool diversity evaluated within bean landraces may help to initiate breeding program for common bean improvement.

摘要

普通菜豆(Phaseolus vulgaris L.)是一种在全球种植的豆类作物,是巴基斯坦山区居民获取蛋白质的非常重要的食物来源。西喜马拉雅山脉拥有丰富的生物多样性,包括未被探索的普通菜豆地方品种。不幸的是,巴基斯坦对这种宝贵作物的关注极少,该国所需的普通菜豆主要从埃塞俄比亚进口。对该地区现有种质资源的开发、利用、保存和繁殖,对于该作物的可持续生产以及提高山区当地社区的营养价值非常重要。利用形态生理和分子标记对来自巴基斯坦自由克什米尔地区和北部地区的普通菜豆地方品种的生物多样性进行了一项研究。从自由克什米尔地区和巴基斯坦北部的不同海拔高度收集了35个普通菜豆生态型以及一个对照品种,并对其生物多样性进行了筛选。形态学特征分析显示,在包括茎花青素、生长类型、开花天数、单株荚数和百粒重等参数方面存在高度遗传多样性。利用SSR标记进行基因组特征分析,以评估种质资源间的等位基因多样性,也呈现出多样的图谱,条带模式的多态性为83.3%。在菜豆地方品种中评估的大量基因库多样性可能有助于启动普通菜豆改良的育种计划。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d404/9079248/6d7c6ca59a5d/gr1.jpg

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