Department of Parasitology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Pathology, Hospital Sungai Buloh, Ministry of Health, Malaysia.
Int J Infect Dis. 2022 Jul;120:132-134. doi: 10.1016/j.ijid.2022.04.036. Epub 2022 Apr 25.
Preventing reverse transcription loop-mediated isothermal amplification (RT-LAMP) carryover contamination could be solved by adding deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) into the reaction master mix.
RNA was extracted from nasopharyngeal swab samples by a simple RNA extraction method.
Testing of 77 samples demonstrated 91.2% sensitivity (95% confidence interval [CI]: 78-98.2%) and 100% specificity (95% confidence interval: 92-100%) using UDG RT-LAMP.
This colorimetric UDG RT-LAMP is a simple-to-use, fast, and easy-to-interpret method, which could serve as an alternative for diagnosis of SARS-CoV-2 infection, especially in remote hospitals and laboratories with under-equipped medical facilities.
通过在反应主混合物中添加脱氧尿苷三磷酸（dUTP）和尿嘧啶-DNA 糖基化酶（UDG），可以解决逆转录环介导等温扩增（RT-LAMP）的携带污染问题。
通过简单的 RNA 提取方法从鼻咽拭子样本中提取 RNA。
使用 UDG RT-LAMP 对 77 个样本进行测试，灵敏度为 91.2%（95%置信区间[CI]：78-98.2%），特异性为 100%（95%置信区间：92-100%）。
这种显色 UDG RT-LAMP 是一种简单易用、快速且易于解释的方法，可作为 SARS-CoV-2 感染诊断的替代方法，尤其是在医疗设施配备不足的偏远医院和实验室。
