School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510300, China.
Phytomedicine. 2022 Jul;101:154101. doi: 10.1016/j.phymed.2022.154101. Epub 2022 Apr 18.
Acne has become one of the most prevalent skin disorders, affecting mostly young people's physical and mental health globally. Cryptotanshinone (CPT) is a potential drug for acne, but its mechanism of acne treatment has not been thoroughly studied on the microbiota. Till date, only a few studies are directed to the impact of acne therapy on skin microbiota and lipid metabolites.
The action mechanism of CPT treatment of acne was investigated by the strategy of microbiome integration with lipidomics.
The 16Sr DNA sequencing was used to detect skin microbiota composition, and absolute quantitative lipidomics was utilized to identify lipid metabolites profiles levels. Four key proteins of the glycolysis pathway were detected with the immunochemistry method. Antibacterial analysis was used to evaluate CPT treatment of acne.
CPT significantly inhibited Staphylococcus epidermidis and Staphylococcus aureus. Combination of the skin microbiome and lipidomics analysis, 29 types of differentially expressed flora (DEFs) and 782 differentially expressed lipid metabolites (DELMs) were significantly altered, especially Staphylococcus, Corynebacterium, Ralstonia, Enhydrobacter, Burkholderia, and Streptococcus. Cer was mainly regulated by Staphylococcus and Corynebacterium, whereas TG and DG were mainly regulated by Ralstonia, Enhydrobacter, Burkholderia, and Streptococcus. The glycolysis pathway was significantly regulated by Staphylococcus on CPT treatment of acne. The energy metabolism, lipid metabolism, immune system, glycan biosynthesis, and metabolism could be reversed by CPT.
CPT might help acne rats rebuild their skin microbiota and alter lipid metabolism signatures. Furthermore, since skin microbes and skin lipid metabolites have a close correlation and are both regulated by CPT, the findings potentially provide a research foundation for the discovery of biomarkers of skin microbiome imbalance and targeted treatment of acne development mechanisms.
痤疮已成为全球最常见的皮肤疾病之一，极大地影响了年轻人的身心健康。隐丹参酮（CPT）是一种有潜力的治疗痤疮的药物，但它在微生物组方面治疗痤疮的机制尚未得到充分研究。迄今为止，只有少数研究针对痤疮治疗对皮肤微生物组和脂质代谢物的影响。
通过微生物组与脂质组学整合的策略研究 CPT 治疗痤疮的作用机制。
采用 16SrDNA 测序检测皮肤微生物群落组成，绝对定量脂质组学检测脂质代谢物谱水平。采用免疫化学方法检测糖酵解途径的 4 种关键蛋白。采用抑菌分析评估 CPT 治疗痤疮的效果。
CPT 能显著抑制表皮葡萄球菌和金黄色葡萄球菌。皮肤微生物组和脂质组学分析相结合，发现 29 种差异表达菌群（DEFs）和 782 种差异表达脂质代谢物（DELMs）显著改变，特别是葡萄球菌属、棒状杆菌属、雷尔氏菌属、 Enhydrobacter、伯克霍尔德氏菌属和链球菌属。蜡酯主要受葡萄球菌属和棒状杆菌属调节，而三酰甘油和二酰基甘油主要受雷尔氏菌属、 Enhydrobacter、伯克霍尔德氏菌属和链球菌属调节。痤疮治疗中糖酵解途径主要受葡萄球菌属调节。CPT 可逆转能量代谢、脂质代谢、免疫系统、聚糖生物合成和代谢。
CPT 可能有助于痤疮大鼠重建皮肤微生物群并改变脂质代谢特征。此外，由于皮肤微生物和皮肤脂质代谢物密切相关，且均受 CPT 调节，这些发现可能为发现皮肤微生物组失衡的生物标志物和针对痤疮发病机制的靶向治疗提供研究基础。
