Imrie A A, Kehrer S, Smith G W, Penny R, Cooper D A
Pathology. 1986 Oct;18(4):438-43. doi: 10.3109/00313028609087565.
One thousand sera shown to be reactive by one of two commercial enzyme linked immunosorbent assays (ELISA) for antibodies to the AIDS virus were referred to the NSW State Reference Laboratory for confirmatory assays. Each serum was retested by two commercial ELISA systems (Abbott and ENI), the ENI exclusionary H9 ELISA and an immunofluorescence assay. Three hundred and twenty four sera were reactive by all 3 tests whereas 244 demonstrated concordant non-reactivity. Three hundred and seventy seven sera were reactive by Abbott EIA only and could not be confirmed positive by the ENI ELISA incorporating exclusionary testing, immunofluorescence or Western immunoblot of representative sera. Sera obtained from teaching hospital laboratories were more likely to be positive and less likely to be negative by all 3 tests, and were also less likely to be Abbott EIA reactive only compared with sera obtained from the blood transfusion service. Of the remaining 55 sera, 52 demonstrated a negative immunofluorescent reaction or a pattern of equal fluorescence on AIDS virus infected and control cells. Representative sera were shown to be negative on Western immunoblot analysis. Of the 3 sera which demonstrated immunofluorescence reactivity, one was positive and one was negative by Western immunoblot, and one could not be determined. We conclude that a combination of two ELISAs, one with an exclusionary ELISA test and an immunofluorescence assay is a reliable and simple means of confirming reactive sera for AIDS virus antibodies.
通过两种用于检测艾滋病病毒抗体的商用酶联免疫吸附测定（ELISA）中的一种显示为阳性反应的1000份血清，被送交新南威尔士州州立参考实验室进行确证检测。每份血清均通过两种商用ELISA系统（雅培和ENI）、ENI排除性H9 ELISA以及免疫荧光测定进行重新检测。324份血清在所有3项检测中均呈阳性反应，而244份血清则显示出一致的阴性反应。377份血清仅在雅培酶免疫测定中呈阳性反应，通过包含排除性检测、免疫荧光或代表性血清的蛋白质印迹法的ENI ELISA无法确认为阳性。与从输血服务机构获得的血清相比，从教学医院实验室获得的血清更有可能在所有3项检测中呈阳性，而不太可能呈阴性，并且也不太可能仅在雅培酶免疫测定中呈阳性反应。在其余55份血清中，52份在艾滋病病毒感染细胞和对照细胞上显示出阴性免疫荧光反应或荧光强度相等的模式。代表性血清在蛋白质印迹分析中显示为阴性。在3份显示免疫荧光阳性反应的血清中，1份在蛋白质印迹分析中呈阳性，1份呈阴性，1份无法确定。我们得出结论，两种ELISA、一种排除性ELISA检测和一种免疫荧光测定相结合，是确证艾滋病病毒抗体反应性血清的可靠且简便的方法。
