Forghani B, Hurst J W, Chan C S
Viral and Rickettsial Disease Laboratory, California State Department of Health Services, Berkeley 94704.
J Clin Microbiol. 1991 Oct;29(10):2266-72. doi: 10.1128/jcm.29.10.2266-2272.1991.
A HeLa T4+ cell line persistently infected with human immunodeficiency virus type 1 (HIV-1) was used in an indirect immunofluorescent antibody assay (IFA) system to explore its potential suitability as an alternative source of viral antigen for confirmatory IFA in HIV serology. In a study of 121 serum samples chosen because they were reactive on repeat examination by enzyme immunoassay but nonspecific by IFA by using HIV-1-infected H9 cells (H9 IFA) or gave discrepant results by enzyme immunoassay and H9 IFA, the specificity and sensitivity of the HeLa T4+ IFA were comparable to those of Western blot (immunoblot), and identification of the true positive samples among these discrepant or nonspecific samples by HeLa T4+ IFA was approximately twice that by H9 IFA. The primary advantages of using the HeLa cell line rather than lymphoid cell lines in IFA are that cells can be grown as a monolayer and that the individual cells are much larger. The cell membrane, cytoplasm, and nucleus are easily discernible; this allows specific and nonspecific staining to be distinguished. At least eight different nonspecific nuclear and cytoplasmic staining patterns were identified in this study by using T4+ cells.
使用一株持续感染1型人类免疫缺陷病毒（HIV-1）的HeLa T4+细胞系，在间接免疫荧光抗体测定（IFA）系统中，探索其作为HIV血清学确证性IFA病毒抗原替代来源的潜在适用性。在一项对121份血清样本的研究中，这些样本因在酶免疫测定重复检测中呈反应性，但使用HIV-1感染的H9细胞进行IFA（H9 IFA）时呈非特异性，或在酶免疫测定和H9 IFA中给出不一致结果而被选取。HeLa T4+ IFA的特异性和敏感性与免疫印迹法相当，并且在这些不一致或非特异性样本中，HeLa T4+ IFA识别真阳性样本的能力约为H9 IFA的两倍。在IFA中使用HeLa细胞系而非淋巴细胞系的主要优点是细胞可以单层生长，且单个细胞大得多。细胞膜、细胞质和细胞核易于辨别；这使得能够区分特异性和非特异性染色。在本研究中，使用T4+细胞鉴定出至少八种不同的非特异性核染色和细胞质染色模式。
