Eskisehir Acibadem Hospital, Obstetrics and Gynecology Clinic, Eskisehir, Turkey.
Department of Biology, Faculty of Science, Eskişehir Technical University, Eskisehir, Turkey.
Anticancer Agents Med Chem. 2022;22(18):3157-3162. doi: 10.2174/1871520622666220426101409.
Cervical cancer is the deadliest gynecological malignancy. This study aims to examine the anticancer effects of L-citrulline on HeLa cell culture.
HeLa cells were cultured in complete Eagle's minimum essential medium. HeLa cells were seeded in 96-well plates and incubated with L-citrulline. After incubation, MTT dye was added and incubated. Annexin- V technique was used to test the apoptosis. The activated caspases of HeLa cells by L-citrulline exposure were measured with the Caspase 3/7 technique. One-way variance analysis was conducted for statistical analysis by using GraphPad Prism 6.0 for Windows.
L-citrulline showed its toxicity on HeLa cells in a dose-dependent manner in application times of 24 and 48 hours. The IC dose of L-citrulline was 0.19 and 0.16 mg/mL at 24 and 48 hours, respectively. When HeLa cells were exposed to an IC dose of L-citrulline for 24 hours, the percentages of the dead, early apoptotic, and late apoptotic cells were detected to be 0.75%, 23.05%, and 12.75%, respectively. The differences in the wideness of the scratch area were observed at the initial stage and after 24 hours of applying L-citrulline.
L-citrulline showed its toxicity on HeLa cells in a dose-dependent manner. Based on Annexin and Caspase findings, it can be concluded that L-citrulline exerted a pro-apoptotic effect on HeLa cells in only a short exposure time. L-citrulline also showed a migration inhibitory effect. The findings of this study indicate L-citrulline to be worthy of investigation for its anticancer activities in vitro and in vivo, and as a candidate for cancer therapy.
宫颈癌是最致命的妇科恶性肿瘤。本研究旨在研究 L-瓜氨酸对 HeLa 细胞培养的抗癌作用。
HeLa 细胞在完全 Eagle 最低必需培养基中培养。HeLa 细胞接种于 96 孔板中，并用 L-瓜氨酸孵育。孵育后，加入 MTT 染料并孵育。用 Annexin-V 技术检测细胞凋亡。用 Caspase 3/7 技术检测 L-瓜氨酸暴露后 HeLa 细胞中激活的半胱天冬酶。使用 GraphPad Prism 6.0 for Windows 进行单向方差分析进行统计分析。
L-瓜氨酸在 24 和 48 小时的应用时间内，以剂量依赖性方式显示出对 HeLa 细胞的毒性。L-瓜氨酸的 IC 剂量分别为 0.19 和 0.16 mg/mL，在 24 和 48 小时时。当 HeLa 细胞暴露于 IC 剂量的 L-瓜氨酸 24 小时时，死亡、早期凋亡和晚期凋亡细胞的百分比分别为 0.75%、23.05%和 12.75%。在用 L-瓜氨酸处理后，在划痕区域的初始阶段和 24 小时后观察到宽度差异。
L-瓜氨酸以剂量依赖性方式显示出对 HeLa 细胞的毒性。基于 Annexin 和 Caspase 的发现，可以得出结论，L-瓜氨酸在短时间暴露时对 HeLa 细胞产生促凋亡作用。L-瓜氨酸还显示出抑制迁移的作用。本研究的结果表明，L-瓜氨酸在体外和体内具有抗癌活性，并且作为癌症治疗的候选物，值得进一步研究。
