Department of Internal Medicine, West Kazakhstan Marat Ospanov Medical University, 68 Maresyev Str., 030019, Aktobe, Republic of Kazakhstan.
Mol Cell Biochem. 2022 Sep;477(9):2249-2255. doi: 10.1007/s11010-022-04440-1. Epub 2022 Apr 26.
The purpose of this study was to investigate the effect of body mass index on DNA damage of lymphocytes in obese patients without carbohydrate metabolism disorder in the Kazakh population. Research design is based on a single-stage descriptive study. The sample included 239 patients aged 18-60 years. Among the participants, people with chronic decompensated diseases, with bad habits (smokers, drug users, drinkers) were excluded. Special attention was paid to the exclusion of diabetes mellitus to exclude the effect of hyperglycemia on DNA damage when forming the sample according to WHO criteria. The following were estimated: the diameter of the breaks (Foci dia, µm), the average number of γ-HAX (n) foci detected per cell. The study of DNA damage of blood lymphocytes in individuals of the Kazakh population showed high rates of DSB with a BMI over 40 kg/m. The number of breaks per cell in women is significantly higher than in men (p = 0.004). The median test revealed a significant difference in the number of DSBs between different age groups (χ = 10.39, p = 0.0155). Obesity is now gaining momentum, so the study of the effect of body mass index on lymphocyte DNA damage in obese patients without impaired carbohydrate metabolism gives valuable results in the treatment of this disease.
本研究旨在探讨哈萨克人群中无碳水化合物代谢紊乱的肥胖患者体重指数对淋巴细胞 DNA 损伤的影响。研究设计基于单阶段描述性研究。样本包括 239 名年龄在 18-60 岁的患者。参与者中，排除患有慢性失代偿性疾病和不良习惯（吸烟者、吸毒者、饮酒者）的人群。根据世界卫生组织的标准，在形成样本时特别注意排除糖尿病，以排除高血糖对 DNA 损伤的影响。评估内容包括：断裂直径（Foci dia，µm）和每个细胞中检测到的 γ-HAX（n）焦点的平均数量。对哈萨克人群血液淋巴细胞 DNA 损伤的研究表明，BMI 超过 40kg/m 时，DSB 的发生率较高。女性细胞内断裂数量明显高于男性（p=0.004）。中位数检验显示不同年龄组之间 DSB 数量存在显著差异（χ²=10.39，p=0.0155）。肥胖症正在迅速发展，因此研究肥胖患者体重指数对无碳水化合物代谢紊乱的淋巴细胞 DNA 损伤的影响，在治疗这种疾病方面具有重要意义。
