Center for Primary Care and Public Health (Unisanté), University of Lausanne, Route de la Corniche, 21066 Epalinges-Lausanne, Switzerland.
Institut national de recherche et de sécurité (INRS), 54000 Nancy, France.
Int J Mol Sci. 2020 May 26;21(11):3743. doi: 10.3390/ijms21113743.
Oxidative stress reflects a disturbance in the balance between the production and accumulation of reactive oxygen species (ROS). ROS are scavenged by the antioxidant system, but when in excess concentration, they can oxidize proteins, lipids, and DNA. DNA damage is usually repaired, and the oxidized products are excreted in urine. 8-hydroxy-2-deoxyguanosine is considered a biomarker for oxidative damage of DNA. It is needed to define background ranges for 8-OHdG, to use it as a measure of oxidative stress overproduction. We established a standardized protocol for a systematic review and meta-analysis to assess background ranges for urinary 8-OHdG concentrations in healthy populations. We computed geometric mean (GM) and geometric standard deviations (GSD) as the basis for the meta-analysis. We retrieved an initial 1246 articles, included 84 articles, and identified 128 study subgroups. We stratified the subgroups by body mass index, gender, and smoking status reported. The pooled GM value for urinary 8-OHdG concentrations in healthy adults with a mean body mass index (BMI) ≤ 25 measured using chemical methods was 3.9 ng/mg creatinine (interquartile range (IQR): 3 to 5.5 ng/mg creatinine). A significant positive association was observed between smoking and urinary 8-OHdG concentrations when measured by chemical analysis. No gender effect was observed.
氧化应激反映了活性氧（ROS）的产生和积累之间平衡的破坏。ROS 可被抗氧化系统清除，但当浓度过高时，它们会氧化蛋白质、脂质和 DNA。DNA 损伤通常会被修复，氧化产物会随尿液排出。8-羟基-2-脱氧鸟苷被认为是 DNA 氧化损伤的生物标志物。需要确定 8-OHdG 的背景范围，将其用作衡量氧化应激过度产生的指标。我们制定了一个标准化方案，用于系统评价和荟萃分析，以评估健康人群尿液 8-OHdG 浓度的背景范围。我们计算了几何平均值（GM）和几何标准差（GSD），作为荟萃分析的基础。我们最初检索到 1246 篇文章，纳入了 84 篇文章，并确定了 128 个研究亚组。我们根据报告的体重指数、性别和吸烟状况对亚组进行分层。使用化学方法测量的 BMI≤25 的健康成年人尿液 8-OHdG 浓度的 GM 值为 3.9ng/mg 肌酐（四分位距（IQR）：3 至 5.5ng/mg 肌酐）。化学分析时，吸烟与尿液 8-OHdG 浓度之间存在显著正相关。未观察到性别影响。
