Kojima Chiaki, Noguchi Akemi, Nagai Tatsuya, Yuyama Ken-Ichi, Fujii Sho, Ueno Kosei, Oyamada Nobuaki, Murakoshi Kei, Shoji Tatsuya, Tsuboi Yasuyuki
Division of Molecular Materials Science, Graduate School of Science, Osaka City University, Sugimoto 3-3-138, Sumiyoshi, Osaka 558-8585, Japan.
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Hokkaido 060-0808, Japan.
ACS Omega. 2022 Apr 5;7(15):13120-13127. doi: 10.1021/acsomega.2c00553. eCollection 2022 Apr 19.
Membrane fusion (MF) is one of the most important and ubiquitous processes in living organisms. In this study, we developed a novel method for MF of liposomes. Our method is based on laser-induced bubble generation on gold surfaces (a plasmonic nanostructure or a flat film). It is a simple and quick process that takes about 1 min. Upon bubble generation, liposomes not only collect and become trapped but also fuse to form long tubes beneath the bubble. Moreover, during laser irradiation, these long tubes remain stable and move with a waving motion while continuing to grow, resulting in the creation of ultralong tubes with lengths of about 50 μm. It should be noted that the morphology of these ultralong tubes is analogous to that of a sea anemone. The behavior of the tubes was also monitored by fluorescence microscopy. The generation of these ultralong tubes is discussed on the basis of Marangoni convection and thermophoresis.
膜融合（MF）是生物体内最重要且最普遍存在的过程之一。在本研究中，我们开发了一种用于脂质体膜融合的新方法。我们的方法基于金表面（等离子体纳米结构或平面薄膜）上激光诱导气泡的产生。这是一个简单快速的过程，大约需要1分钟。气泡产生时，脂质体不仅会聚集并被困住，还会融合形成气泡下方的长管。此外，在激光照射期间，这些长管保持稳定并以波动运动移动，同时继续生长，从而形成长度约为50μm的超长管。需要注意的是，这些超长管的形态类似于海葵。还通过荧光显微镜监测了这些管的行为。基于马兰戈尼对流和热泳对这些超长管的产生进行了讨论。
