Brain Function Laboratory, National Institute of Genetics, 1111 Yata, Mishima 411-8540, Japan.
The Graduate University for Advanced Studies, SOKENDAI, Hayama, Japan.
Cell Rep Methods. 2021 May 25;1(3):100012. doi: 10.1016/j.crmeth.2021.100012. eCollection 2021 Jul 26.
Neuronal birthdate is one of the major determinants of neuronal phenotypes. However, most birthdating methods are retrospective in nature, allowing very little experimental access to the classified neuronal subsets. Here, we introduce four neurogenic tagging mouse lines, which can assign CreER- recombination to neuron subsets that share the same differentiation timing in living animals and enable various experimental manipulations of the classified subsets. We constructed a brain atlas of the neurogenic tagging mouse lines (NeuroGT), which includes holistic image data of the -recombined neurons and their processes across the entire brain that were tagged on each single day during the neurodevelopmental period. This image database, which is open to the public, offers investigators the opportunity to find specific neurogenic tagging driver lines and the stages of tagging appropriate for their own research purposes.
神经元的出生日期是神经元表型的主要决定因素之一。然而，大多数标记出生日期的方法本质上是回顾性的，对分类神经元亚群的实验研究几乎没有任何帮助。在这里，我们介绍了四种神经发生标记的小鼠品系，它们可以将 CreER 重组分配给具有相同分化时间的神经元亚群，从而在活体动物中对分类亚群进行各种实验操作。我们构建了神经发生标记小鼠品系的脑图谱（NeuroGT），其中包括在神经发育期间的每一天对整个大脑中的 - 重组神经元及其过程进行整体成像数据。这个开放给公众的图像数据库为研究人员提供了寻找特定的神经发生标记驱动线和适合他们自己研究目的的标记阶段的机会。
