Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, 69120 Heidelberg, Germany.
National Center for Tumor Diseases, German Cancer Consortium, Heidelberg Institute of Radiation Oncology and National Center for Radiation Oncology, 69120 Heidelberg, Germany.
Cell Rep Methods. 2022 Feb 17;2(2):100169. doi: 10.1016/j.crmeth.2022.100169. eCollection 2022 Feb 28.
Clonogenic survival assay constitutes the gold standard method for quantifying radiobiological effects. However, it neglects cellular radiation response variability and heterogeneous energy deposition by ion beams on the microscopic scale. We introduce "Cell-Fit-HD" a biosensor that enables a deconvolution of individual cell fate in response to the microscopic energy deposition as visualized by optical microscopy. Cell-Fit-HD enables single-cell dosimetry in clinically relevant complex radiation fields by correlating microscopic beam parameters with biological endpoints. Decrypting the ion beam's energy deposition and molecular effects at the single-cell level has the potential to improve our understanding of radiobiological dose concepts as well as radiobiological study approaches in general.
集落形成存活分析是量化放射生物学效应的金标准方法。然而，它忽略了细胞对辐射的反应变异性，以及离子束在微观尺度上不均匀的能量沉积。我们引入了“Cell-Fit-HD”，这是一种生物传感器，可以通过光学显微镜可视化来解卷积单个细胞对微观能量沉积的反应。通过将微观束参数与生物学终点相关联，Cell-Fit-HD 能够在临床上相关的复杂辐射场中进行单细胞剂量测定。解析离子束在单细胞水平上的能量沉积和分子效应有可能提高我们对放射生物学剂量概念的理解，以及对一般放射生物学研究方法的理解。
