Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, 69120 Heidelberg, Germany; National Center for Tumor Diseases, German Cancer Consortium, Heidelberg Institute of Radiation Oncology and National Center for Radiation Oncology, 69120 Heidelberg, Germany.
Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, 69120 Heidelberg, Germany; National Center for Tumor Diseases, German Cancer Consortium, Heidelberg Institute of Radiation Oncology and National Center for Radiation Oncology, 69120 Heidelberg, Germany.
STAR Protoc. 2022 Oct 29;3(4):101798. doi: 10.1016/j.xpro.2022.101798. eCollection 2022 Dec 16.
We present a protocol for the biosensor Cell-Fit-HD. It enables long-term monitoring and correlation of single-cell fate with subcellular-deposited energy of ionizing radiation. Cell fate tracking using widefield time-lapse microscopy is uncoupled in time from confocal ion track imaging. Registration of both image acquisition steps allows precise ion track assignment to cells and correlation with cellular readouts. For complete details on the use and execution of this protocol, please refer to Niklas et al. (2022).
我们提出了一种 Cell-Fit-HD 生物传感器的使用方案。该方案可实现对单细胞命运与电离辐射亚细胞沉积能量的长期监测和关联。使用宽场延时显微镜进行细胞命运跟踪在时间上与共聚焦离子径迹成像解耦。这两个图像采集步骤的注册允许将精确的离子径迹分配给细胞，并与细胞读出结果相关联。有关此方案使用和执行的完整详细信息，请参阅 Niklas 等人（2022 年）。
