Erukainure Ochuko L, Rademan Sunelle, Erhabor Joseph O, Chukwuma Chika I, Nde Adeline Lum, Matsabisa Motlalepula G
Department of Pharmacology, School of Clinical Medicine, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
Department of Pharmacology, School of Clinical Medicine, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa; Phytomedicine Unit, Department of Plant Biology and Biotechnology, University of Benin, Benin City, Nigeria.
J Ethnopharmacol. 2022 Jul 15;293:115312. doi: 10.1016/j.jep.2022.115312. Epub 2022 Apr 26.
Cannabis sativa L. is among numerous medicinal plants widely used in traditional medicine in treating various ailments including kidney diseases.
The protective effect of C. sativa on oxidative stress, cholinergic and purinergic dysfunctions, and dysregulated glucogenic activities were investigated in oxidative injured kidney (Vero) cell lines.
Fixed Vero cells were treated with sequential extracts (hexane, dichloromethane [DCM] and ethanol) of C. sativa leaves for 48 h before subjecting to MTT assay. Vero cells were further incubated with FeSO for 30 min, following pretreatment with C. sativa extracts for 25 min. Normal control consisted of Vero cells not treated with the extracts and/or FeSO, while untreated (negative) control consisted of cells treated with only FeSO.
MTT assay revealed the extracts were slightly cytotoxic at the highest concentrations (250 μg/mL). There was a significant depletion in glutathione level and catalase activity on induction of oxidative stress, with significant elevation in malondialdehyde level, acetylcholinesterase, ATPase, ENTPDase, fructose-1,6-biphosphatase, glucose 6-phosphatase and glycogen phosphorylase activities. These activities and levels were significantly reversed following pretreatment with C. sativa extracts.
These results portray the protective potentials of C. sativa against iron-mediated oxidative renal injury as depicted by the ability of its extracts to mitigate redox imbalance and suppress acetylcholinestererase activity, while concomitantly modulating purinergic and glucogenic enzymes activities in Vero cells.
大麻是广泛用于传统医学治疗包括肾脏疾病在内的各种疾病的众多药用植物之一。
在氧化损伤的肾（Vero）细胞系中研究大麻对氧化应激、胆碱能和嘌呤能功能障碍以及糖原异生活动失调的保护作用。
在进行MTT试验前，将固定的Vero细胞用大麻叶的顺序提取物（己烷、二氯甲烷[DCM]和乙醇）处理48小时。在用大麻提取物预处理25分钟后，将Vero细胞与硫酸亚铁进一步孵育30分钟。正常对照由未用提取物和/或硫酸亚铁处理的Vero细胞组成，而未处理（阴性）对照由仅用硫酸亚铁处理的细胞组成。
MTT试验显示，提取物在最高浓度（250μg/mL）时具有轻微细胞毒性。氧化应激诱导后，谷胱甘肽水平和过氧化氢酶活性显著降低，丙二醛水平、乙酰胆碱酯酶、ATP酶、ENTPD酶、果糖-1,6-二磷酸酶、葡萄糖6-磷酸酶和糖原磷酸化酶活性显著升高。用大麻提取物预处理后，这些活性和水平显著逆转。
这些结果表明，大麻提取物能够减轻氧化还原失衡、抑制乙酰胆碱酯酶活性，同时调节Vero细胞中嘌呤能和糖原异生酶的活性，从而展现出其对铁介导的氧化肾损伤的保护潜力。
