National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Environmental Microbiome and Biotechnology Lab, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.
Bioresour Technol. 2022 Jun;354:127227. doi: 10.1016/j.biortech.2022.127227. Epub 2022 Apr 25.
To take full advantage of refractory dissolved organic matters (rDOMs) and generate sufficient nitrate for domestic sewage treatment, this study presented a novel integrated fermentation, nitrification, denitratation and anammox (IFNDA) process in a combined ABR-CSTR reactor. The results showed that an advanced total nitrogen (TN) removal efficiency of 94.1% was obtained after over 190 days operation, resulting in effluent TN concentration as low as 3.6 mg/L. The system nitrogen removal was dominated by anammox with a high proportion of 88.6%. The high conversion rate of acetic acid (54.0%) and volatile fatty acids (64.5%) from rDOMs in domestic sewage by in-situ fermentation drove efficient denitratation. Microflora analysis indicated that the enriched Commamonas (3.5%) and Longilinea (3.3%) dominated hydrolysis and acidogenesis of organics, and Methanosaeta (9.0%) obligated acetoclastic methanogenesis in two-stage fermentation process. Thauera (8.4%) and Candidatus Brocadia (2.5%) were the core bacteria for nitrogen metabolism in the IFNDA system.
为充分利用难生物降解溶解性有机物（rDOMs）并生成足够的硝酸盐以用于处理生活污水，本研究在一个组合式 AB反应器-连续流搅拌槽式反应器（CSTR）中提出了一种新颖的集成发酵、硝化、反硝化和厌氧氨氧化（IFNDA）工艺。结果表明，经过 190 多天的运行，该系统获得了高达 94.1%的先进总氮（TN）去除效率，出水 TN 浓度低至 3.6mg/L。该系统的脱氮主要由氨氧化作用主导，其比例高达 88.6%。生活污水中 rDOMs 中的乙酸（54.0%）和挥发性脂肪酸（64.5%）经原位发酵得到了高转化率，从而推动了高效反硝化作用。微生物群落分析表明，富集的 Comamonas（3.5%）和 Longilinea（3.3%）主导有机物的水解和产酸，而 Methanosaeta（9.0%）在两段式发酵过程中负责乙酸型产甲烷作用。Thauera（8.4%）和 Candidatus Brocadia（2.5%）是 IFNDA 系统中氮代谢的核心细菌。
