Yuan Bo, Chen Yang, Yuan Jingyan, Zeng Lizhong, Yang Shuanying
Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Zhongguo Fei Ai Za Zhi. 2022 Apr 20;25(4):226-235. doi: 10.3779/j.issn.1009-3419.2022.102.05.
A lack of effective treatment for lung squamous cell carcinoma (LUSC) makes it an important factor restricting the 5-year survival rate of non-small cell lung cancer (NSCLC). Long non-coding RNA 00668 (LINC00668) was reported to play crucial regulatory roles in the tumorigenesis and progression of various cancers; however, its role in LUSC is unclear. The aim of this study was to investigate the prognosis value and biological function of LINC00668 in NSCLC, especially in LUSC.
The expression pattern of LINC00668 and its relationship with clinical characteristics and prognosis of patients were investigated in the NSCLC especially LUSC based on The Cancer Genome Altas (TCGA) database. Its function in LUSC cells was explored in vitro.
LINC00668 expression was significantly up-regulated in LUSC patients and high expression level of LINC00668 was associated with advanced tumor-node-metastasis (TMN) stage. Moreover, the expression of LINC00668 significantly increased in smoking patients, and was a prognostic indicator for overall survival (OS) of smoking patients with LUSC. In vitro experiments showed that LINC00668 has significantly higher expression level in LUSC cell lines and tissues compared to normal bronchial epithelial cell and para-tumor tissues; meanwhile, functional assay indicated knockdown of LINC00668 effectively inhibited the migration and invasion of LUSC cells.
LINC00668 might closely relate to the development of LUSC, and inhibition of LINC00668 may reduce the metastasis of LUSC.
缺乏有效的肺鳞状细胞癌（LUSC）治疗方法是限制非小细胞肺癌（NSCLC）5年生存率的重要因素。据报道，长链非编码RNA 00668（LINC00668）在多种癌症的发生和发展中起关键调节作用；然而，其在LUSC中的作用尚不清楚。本研究旨在探讨LINC00668在NSCLC，尤其是LUSC中的预后价值和生物学功能。
基于癌症基因组图谱（TCGA）数据库，研究NSCLC尤其是LUSC中LINC00668的表达模式及其与患者临床特征和预后的关系。在体外探索其在LUSC细胞中的功能。
LINC00668在LUSC患者中表达显著上调，且LINC00668高表达与晚期肿瘤-淋巴结-转移（TMN）分期相关。此外，LINC00668在吸烟患者中表达显著增加，是吸烟LUSC患者总生存期（OS）的预后指标。体外实验表明，与正常支气管上皮细胞和癌旁组织相比，LINC00668在LUSC细胞系和组织中的表达水平显著更高；同时，功能分析表明敲低LINC00668可有效抑制LUSC细胞的迁移和侵袭。
LINC00668可能与LUSC的发生密切相关，抑制LINC00668可能减少LUSC的转移。
