Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Jordan Dermatology and Hair Transplantation Center, Tehran, Iran.
J Cosmet Dermatol. 2022 Oct;21(10):5156-5167. doi: 10.1111/jocd.15022. Epub 2022 May 10.
Cell-based therapy utilizing mesenchymal stem cells (MSCs) is currently being investigated as a therapeutic agent for chronic wounds. There is no evidence regarding effectiveness of the spray and local transfer of this cellular product in diabetic wound healing. Accordingly, the present study, using clinical, pathological and biometric parameters, investigated the effectiveness of the spray of these cells in the healing of diabetic wounds in rats.
Three days after the induction of diabetes (50 mg/kg single dose of streptozotocin) a circular excision was created on the back of rats. Diabetic rats were divided into two groups (n = 21): Control and WJ-MSCs group. Sampling of the studied groups was performed on Days 7, 14, and 21 after wounding. Histological, ultrasound imaging of dermis and epidermis in the wound area for thickness and density measurement and skin elasticity were evaluated.
Our results on Days 7, 14, and 21 after wounding showed that the wound closure, thickness, and density of new epidermis and dermis, as well as skin elasticity in healed wound were significantly higher in WJ-MSCs group compared with the Control group.
Application of WJ-MSCs suspension spray on the wound area can accelerate healing in diabetic wounds. Our findings may potentially provide a helpful therapeutic strategy for patients with a diabetic wound.
利用间充质干细胞（MSCs）的细胞疗法目前正在被研究作为治疗慢性伤口的一种治疗剂。目前还没有关于这种细胞产品的喷雾和局部转移在糖尿病伤口愈合中有效性的证据。因此，本研究使用临床、病理和生物计量学参数，调查了喷雾这些细胞在糖尿病大鼠伤口愈合中的有效性。
在诱导糖尿病后 3 天（单次给予 50mg/kg 链脲佐菌素），在大鼠背部创建一个圆形切除物。糖尿病大鼠被分为两组（n=21）：对照组和 WJ-MSCs 组。在创伤后第 7、14 和 21 天对研究组进行采样。评估了组织学、伤口区域表皮和真皮的超声成像以测量厚度和密度以及皮肤弹性。
我们在创伤后第 7、14 和 21 天的结果表明，与对照组相比，WJ-MSCs 组的伤口闭合、新表皮和真皮的厚度和密度以及愈合伤口的皮肤弹性显著更高。
WJ-MSCs 混悬液喷雾应用于伤口区域可以加速糖尿病伤口的愈合。我们的发现可能为糖尿病伤口患者提供一种有帮助的治疗策略。
