Departamento de Medio Ambiente y Agronomía, INIA-CSIC, Madrid, Spain.
J Sep Sci. 2022 Jul;45(13):2356-2365. doi: 10.1002/jssc.202200129. Epub 2022 May 3.
In this work, the preparation and evaluation of water-compatible molecularly imprinted polymers for triazines using 2-hydroxyethyl methacrylate and methacrylic acid as comonomers is described. Four sets of molecularly imprinted and non-imprinted polymers for propazine were prepared at varying monomer molar ratios (from 4:0 to 1:3), and evaluated for the recognition of several triazines directly in aqueous media. The evaluation was performed by loading 1 mL of an aqueous solution containing 500 ng of each selected triazine, washing with 500 μL of acetonitrile, and eluting with 500 μL of methanol followed by 2 × 500 μL of a solution of methanol containing 10% of acetic acid. Final determinations were performed by high-performance liquid chromatography-ultraviolet detection. Improvement in molecular recognition of triazines in water was obtained on those molecularly imprinted polymers incorporating 2-hydroxyethyl methacrylate in 3:1 or 2:2 molar ratios, being the former selected as optimum providing recoveries for propazine up to 80%. A molecularly imprinted solid-phase extraction protocol was developed to ensure that triazines-selective recognition takes place inside selective binding sites in pure water media. Finally, the developed method was successfully applied to the determination of the selected triazines in environmental waters providing limits of detection from 0.16 and the 0.5 μg/L concentration range.
在这项工作中，描述了使用 2-羟乙基甲基丙烯酸酯和甲基丙烯酸作为共聚单体制备和评价用于三嗪的水相容的分子印迹聚合物。在不同的单体摩尔比（从 4:0 到 1:3）下，制备了四组用于普草津的分子印迹和非印迹聚合物，并直接在水介质中评价了它们对几种三嗪的识别能力。通过加载 1 mL 含有 500 ng 每种选定三嗪的水溶液，用 500 μL 乙腈洗涤，用 500 μL 甲醇洗脱，然后用 2×500 μL 含有 10%乙酸的甲醇溶液进行洗脱，最后通过高效液相色谱-紫外检测进行最终测定。在含有 2-羟乙基甲基丙烯酸酯摩尔比为 3:1 或 2:2 的分子印迹聚合物中，三嗪在水中的分子识别得到了改善，前者被选为最佳选择，提供了高达 80%的普草津回收率。开发了一种分子印迹固相萃取方案，以确保在纯水介质中的选择性结合位点中发生三嗪选择性识别。最后，该方法成功应用于环境水样中选定的三嗪的测定，检测限为 0.16 至 0.5 μg/L。
