Department of Biomedical Sciences, University of Padova, Padova, Italy.
Veneto Institute of Molecular Medicine, Padova, Italy.
STAR Protoc. 2022 Apr 15;3(2):101322. doi: 10.1016/j.xpro.2022.101322. eCollection 2022 Jun 17.
Mitochondrial respiratory chain (MRC) dysfunction is linked to mitochondrial disease as well as other common conditions such as diabetes, neurodegeneration, cancer, and aging. Thus, the evaluation of MRC enzymatic activities is fundamental for diagnostics and research purposes on experimental models. Here, we provide a verified and reliable protocol for mitochondria isolation from various samples and subsequent measurement of the activity of MRC complexes I-V plus citrate synthase (CS) through UV-VIS spectrophotometry. For complete details on the use and execution of this protocol, please refer to Brischigliaro et al. (2021).
线粒体呼吸链（MRC）功能障碍与线粒体疾病以及其他常见疾病有关，如糖尿病、神经退行性疾病、癌症和衰老。因此，评估 MRC 酶活性对于诊断和实验模型的研究目的至关重要。在这里，我们提供了一种经过验证和可靠的从各种样本中分离线粒体并通过紫外可见分光光度法测量 MRC 复合物 I-V 和柠檬酸合酶（CS）活性的方案。有关该方案使用和执行的完整详细信息，请参阅 Brischigliaro 等人（2021 年）。
