Morel F, Mazet F, Touraille S, Alziari S
Equipe Génome mitochondrial, Université Blaise Pascal-Clermont II, Aubière, France.
Mech Ageing Dev. 1995 Oct 27;84(3):171-81. doi: 10.1016/0047-6374(95)01653-8.
The time course (age 0-8 weeks) of the enzyme activities of respiratory chain complexes I, III and IV and of citrate synthase, and the cell mitochondrial/nuclear DNA content ratio were studied in Drosophila subobscura. The activities of the three respiratory complexes decreased with age, but with different kinetics. The activities of complexes I and III remained nearly stable between weeks 0 and 3 (falling by 6% and 15%, respectively), and then gradually decreased; after 8 weeks residual activities were about 50% of the initial value for complexes I and III. The activity of complex IV fell in the first week, decreasing continually to week 8, where residual activity was 30% of the initial value. No significant age-related change in citrate synthase activity was observed. Mitochondrial DNA (measured by mitDNA/nucDNA) increased linearly up to week 5 (2.6-fold) and then dropped by 40% in week 6 though it remained higher than initial values.
研究了果蝇（Drosophila subobscura）呼吸链复合体I、III和IV以及柠檬酸合酶的酶活性随时间的变化过程（0至8周龄），以及细胞线粒体/核DNA含量比。三种呼吸复合体的活性随年龄增长而下降，但动力学不同。复合体I和III的活性在第0周和第3周之间几乎保持稳定（分别下降6%和15%），然后逐渐下降；8周后，复合体I和III的残余活性约为初始值的50%。复合体IV的活性在第一周下降，持续下降至第8周，此时残余活性为初始值的30%。未观察到柠檬酸合酶活性与年龄相关的显著变化。线粒体DNA（通过mitDNA/nucDNA测量）在第5周前呈线性增加（2.6倍），然后在第6周下降40%，尽管仍高于初始值。
