Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
Department of Otorhinolaryngology, Head and Neck Surgery, School of Medical Sciences, Hospital Universiti Sains Malaysia, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
PeerJ. 2022 Apr 22;10:e13314. doi: 10.7717/peerj.13314. eCollection 2022.
Allergic rhinitis (AR) is a common allergic disease characterized by disruption of nasal epithelial barrier. In this study, we investigated the mRNA expression of zonula occludens-1 (), and and histone deacetylase 1 () and in AR patients compared to healthy controls. RNA samples were extracted from nasal epithelial cells of house dust mites (HDMs)-sensitized AR patients and healthy controls ( = 28 in each group). The RNAs were reverse transcribed into cDNAs for measurement of , , , and expression levels by quantitative PCR. The mRNA expression of was significantly decreased in AR patients compared to healthy controls ( = 0.010). No significant difference was observed in the expression levels of , , and in AR patients compared to healthy controls. We found significant associations of higher levels in AR patients with lower frequency of changing bedsheet ( = 0.043) and with AR patients sensitized to ( = 0.041). Higher expression of was observed in AR patients who had pets ( = 0.007). In conclusion, our data indicated that expression was lower in AR patients contributing to decreased integrity of nasal epithelial barrier integrity, and HDAC2 may be involved in the pathogenesis of the disease.
变应性鼻炎 (AR) 是一种常见的过敏性疾病，其特征是鼻上皮屏障功能障碍。在这项研究中，我们比较了屋尘螨 (HDM) 致敏的 AR 患者和健康对照者鼻上皮细胞中紧密连接蛋白 1 ()、 和组蛋白去乙酰化酶 1 () 和 () 的 mRNA 表达。从 HDM 致敏的 AR 患者和健康对照者 (每组 28 例) 的鼻上皮细胞中提取 RNA 样本。将 RNA 逆转录为 cDNA，通过定量 PCR 测量 、 、 、 和 的表达水平。与健康对照组相比，AR 患者的 表达显著降低 (=0.010)。与健康对照组相比，AR 患者中 、 、 和 的表达水平无显著差异。我们发现 AR 患者中 水平较高与换床单频率较低 (=0.043) 和对 过敏有关 (=0.041)。在有宠物的 AR 患者中观察到 表达较高 (=0.007)。总之，我们的数据表明，AR 患者的 表达降低，导致鼻上皮屏障完整性降低，HDAC2 可能参与了疾病的发病机制。
